research

Research

Kshitiz is an Associate Research Scientist in the Institute of Systems Biology at Yale University, West Haven, CT. He is also an Investigator in the Cancer Systems Biology @ Yale (CaSB@Yale), a U54 center initiated by the National Cancer Institute.

He pursued his PhD from the Department of Biomedical Engineering at the Johns Johns Hopkins School of Medicine, Baltimore, and post doctoral training in the Department of Bioengineering, University of Washington, Seattle. 
Kshitiz's interest ranges largely in understanding how cells interact with their immediate microenvironment, with a particular emphasis on the mechanical and physical aspects on the microenvironment. His recent work has been focused on developing methods to create a heart tissue from human cells for drug screening, drug discovery, and tissue engineering purposes. In addition, he works on understanding the mechanisms by which stem cells and cancer cells respond to the mechanical rigidity, and topography of their milieu, in addition to the physical factors, like oxygen availability. 




Cover Articles
          
     

Publications
(* Authors contributed equally to the manuscript)

23. Kshitiz, Afzal J*, Suhail Y*, Ahn EH, Goyal R, Hubbi ME, Hussaini Q, Ellison DD, Goyal J, Nacev B, Kim DH, Lee JH, Frankel S, Gray K, Bankoti R, Chien AJ, Levchenko A (2015). Control of the interface between heterotypic cell populations reveals the mechanism of intercellular transfer of signaling proteins, Integrative Biology, 7, 364-372. Article

22. Kshitiz, Afzal J, Kim SY, Kim DH (2014). A nanotopography approach for studying the structure-function relationship of cells and tissues, Cell Adhesion & Migration, 8(4). Article

21. Hubbi ME, Gilkes DM, Hu H, Kshitiz, Ahmed I, Semenza GL (2014). Cyclin-dependent kinases regulate lysosomal degradation of hypoxia-inducible factor 1α to promote cell-cycle progression Supporting Information, Proceedings of the National Academy of Sciences (Accepted). Article

20. Kshitiz, Afzal J, Kim DH, Levchenko A (2014). Mechanotransduction via p190RhoGAP regulates a switch between cardiomyogenic and endothelial lineages in adult cardiac progenitors, Stem Cells,  32(8), 1999-2007.  Article

19. Ahn EH*, Kim Y*, Kshitiz*, An SS, Afzal J, Lee S, Kwak M, Suh KY, Kim DH, Levchenko A (2014). Spatial control of stem cell fate using nanotopographic cues, Biomaterials, 35(8), 2401-2410. Article

18. Hubbi ME, Hu H, Kshitiz, Ahmed I, Levchenko A, Semenza GL, (2013). Sirtuin-7 Inhibits the Activity of Hypoxia-Inducible Factors, Journal of Biological Chemistry, 288, 20768-775. Article

17. Kshitiz, Afzal J, Kim SY, Kim DH, (2014). A nanotopography approach for studying the structure-function relationships of cells and tissues, Cell Adhesion and Migration, 8(4). Article 

16. Hubbi ME, Hu H, Kshitiz, Ahmed I, Levchenko A, Semenza GL (2013). Chaperone-mediated autophagy targets HIF-1a for lysosomal degradation, Journal of Cell Biology, (Accepted). Article

15. Hubbi ME*, Kshitiz*, Gilkes DM, Rey S, Wong CC, Luo W, Kim DH, Dang CV, Levchenko A, Semenza GL (2013). A non-transcriptional role for HIF-1a as a direct inhibitor of DNA replication, Science Signaling, vol. 6, pp. ra10. (Featured as a Cover Article). Article Podcast

14. Suhail Y*, Kshitiz*, Lee J, Walker M, Kim DH, Brennan MD, Bader JS, Levchenko A (2013). Modeling intercellular transfer of biomolecules through tunneling nanotubes, Bulletin of Mathematical Biology, 75(8), 1400-1416Article

13. Chaturvedi P, Gilkes DM, Wong CC, Kshitiz, Luo W, Zhang H, Wei H, Takano N, Schito L, Levchenko A, Semenza GL. (2013). Hypoxia-inducible factor-dependent breast cancer-mesenchymal stem cell bidirectional signaling promotes metastasis, Journal of Clinical Investigations, 123(1):189-205. Article

12. Kshitiz*, Hubbi ME*, Ahn EH, Downey J, Kim DH, Rey S, Kundu A, Semenza GL, Abraham RM, Levchenko,
A. (2012). Matrix rigidity coordinates the proliferation, differentiation, and morphogenesis of endothelial cells
derived from cardiac progenitors, Science Signaling, 5(227):ra41. (Featured as a ArticleHighlighted in Science). Article

11. Kim DH*, Kshitiz*, Smith R, Kim P, Marban E, Suh KY, Levchenko A. (2012). Nanopatterned Cardiac Cell Patches
Promote Stem Cell Niche Formation and Myocardial Regeneration, Integrative Biology, 4(9):1019-33. (Featured as a Cover Article). Article

10. Kshitiz, Park JS, Kim P, Helen W, Engler AJ, Levchenko A, Kim DH. (2012). Control of stem cell fate and
function by engineering physical microenvironment, Integrative Biology, 4:1008-18. Article

9. Berman SMC, Kshitiz, Wang JC, Orukari I, Levchenko A, Bulte JWM, Walczak P. (2012). Cell motility of neural stem cells is reduced after SPIO-labeling, which is mitigated after exocytosis, Magnetic Resonance in Imaging, doi: 10.1002/mrm.24216. Article

8. Bankoti R, Gupta K, Levchenko A, Stager S. (2012). Marginal zone B-cells regulate antigen-specific T-cell
responses during infection, Journal of Immunology, 188:3961-71. Article

7. Kshitiz, Kim DH, Beebe D, Levchenko A. (2011). Micro- and nanoengineering for stem cell biology: the
promise with a caution, Trends in Biotechnology, 29(8):399-408. Article

6. Gupta K, Kim DH, Ellison D, Smith C, Kundu A, Tuan J, Suh KY, Levchenko A. (2011). Lab-on- a-chip
devices as an emerging platform for stem cell biology, Lab on a Chip, 10(16):2019-31. Article

5. Rey S, Lee K, Wang CJ, Gupta K, Chen S, McMillan A, Bhise N, Levchenko A, Semenza GL.
(2009). Synergistic effect of HIF-1alpha gene therapy and HIF-1-activated bone marrow-derived angiogenic
cells in a mouse model of limb ischemia, Proc Natl Acad Sci USA. 106(48):20399-404. Article

4. Kim DH, Han K, Gupta K, Kwon KW, Suh KY, Levchenko A. (2009). Mechanosensitivity of fibroblast cell
shape and movement to anisotropic substratum topography gradients, Biomaterials. 30(29):5433-44. Article

3. Niu X, Gupta K, Yang JT, Shamblott MJ, Levchenko A. (2009). Physical transfer of membrane and
cytoplasmic components as a general mechanism of cell-cell communication, Journal of Cell Science, 122(Pt 5):600-610. Article

2. Gupta K*, Sehgal V*, Levchenko A. (2008). A method for probabilistic mapping between
protein structure and function taxonomies through cross training, BMC Structural Biology, 8:40. Article

1. Gupta K, Thomas D, Vidya SV, Venkatesh KV, Ramakumar S. (2005). Detailed protein sequence alignment
based on Spectral Similarity Score (SSS), BMC Bioinformatics, 6:105. Article

 











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