BASIC INFORMATION

Here you will find some basic information on the microscopes and other instruments offered for use in the KINSC.  This information includes model names and specifications for the various scopes, as well as links to the manufacturer and to outside information on how the type of scope works. 

Summary of the Equipment


Cell Sorter

Our cell sorter is a BD Biosciences FACS Aria II system with the ability to sort at 30,000 cells per second, and the capacity to assess up to 12 fluorescent parameters.

        Manufacturer's site [Link Here]

        How Fluorescence Activated Cell Sorting (FACS) works [LinkHere]

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Confocal Microscope

We have a Nikon C1 Confocal on a Nikon Eclipse 80i upright microscope. There are 3 confocal fluorescence channels (405nm, 488nm and 543nm) with associated lasers (Melles Griot lasers: 408 56RCS/S2780 modulated diode laser; Argon Ion Laser model 35-IMA 410-015; and HeNe 05-LGP-193 laser). The associated software includes Nikon’s EZC1 acquisition and analysis software and NIS Elements.

        Manufacturer's site [Link Here]

        How a confocal microscope works [Link Here]

        General microscopy reference http://www.microscopyu.com

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Scanning Electron Microscope (SEM)

Our SEM is a JEOL JSM-6510LV/GS Scanning Electron Microscope, with a resolution of 3.0 nm (at 30kV) in High Vacuum mode and 4.0 nm (at 30kV) in Low Vacuum mode. The accelerating voltage can be varied from 0.5 to 30 kV and the magnification range is 5x to 300,000x. Software includes the Olympus Scandium software.

For sample preparation we also have a Denton Vacuum Desk V Sputter Coater equipped with the ability to rotate the sample during sputtering for a more even coating. We do not have the etch option enabled on this Desk V. The Sputter coater is currently equipped with a gold target but there is the possibility to change this out with your target of choice.

The JSM-6510LV/GS is also equipped with a Nabity Nano Pattern Generation (NPGS) Electron Beam Lithography (EBL) System.

        Manufacturer's site [Link Here]

        How a scanning electron microscope (SEM) works [Link Here]

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Transmission Electron Microscope (TEM)

JEOL JEM-1400 Transmission Electron Microscope, with integrated graphical user interface JENIE (JEOL Electron microscope Navigation Interactive Engine).

        Manufacturer's site [Link Here]

        How a transmission electron microscope (TEM) works [Link Here]

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Compound and Dissecting Microscopes

In our research and teaching labs we have a variety of both compound and dissecting microscopes. These include two Nikon Eclipse 80i upright scopes (Dr Karl Johnson), and several Nikon SMZ1000 dissecting scopes (Drs Rachel Hoang and Andrea Morris). The Eclipse 80i scopes and one SMZ dissecting scope are equipped with Mercury lamps and filters suitable for imaging DAPI/Hoechst, FITC/Alexa 488, and Rhodamine/Alexa 546.

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Immunofluorescence Microscopes

Details coming soon!

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