Immunofluorescence Microscope

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The following 5 java files can be used to place a graticule (rectangular grid) over any image in ImageJ. The grid is calibrated for the Compound Light Microscope in L004 and the Immunofluorescence Microscopes in L006. To use the plugin: Download all 5 java files and place them in a folder labeled Graticule. Then open up the file location for ImageJ, usually C://programs/ImageJ/Plugins for windows and finder>applications>ImageJ>Plugins for mac. Place the Graticule folder in the Plugins folder. Exit out of windows explorer or finder. Open ImageJ. Open a picture. Choose Plugins>Add Graticule.   1k v. 3 Jul 3, 2012, 8:41 AM KINSC Scopes Hoang
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Image of stage micrometer 10um/division at 100x using the Nikon Eclipse 80i IMF Microscope. Use this image to calibrate image or other image processing software in order to perform measurements, add scale bars, etc.  4276k v. 2 Oct 24, 2011, 11:45 AM KINSC Scopes Hoang
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Image of stage micrometer 10um/division at 10x using the Nikon Eclipse 80i IMF Microscope. Use this image to calibrate image or other image processing software in order to perform measurements, add scale bars, etc.  1088k v. 2 Oct 24, 2011, 11:45 AM KINSC Scopes Hoang
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Image of stage micrometer 10um/division at 20x using the Nikon Eclipse 80i IMF Microscope. Use this image to calibrate image or other image processing software in order to perform measurements, add scale bars, etc.  734k v. 3 Oct 24, 2011, 11:45 AM KINSC Scopes Hoang
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Image of stage micrometer 10um/division at 40x using the Nikon Eclipse 80i IMF Microscope. Use this image to calibrate image or other image processing software in order to perform measurements, add scale bars, etc.  1088k v. 2 Oct 24, 2011, 11:45 AM KINSC Scopes Hoang
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Follow instructions for Making Measurements Using ImageJ in the Protocols section  5626k v. 5 Jun 25, 2012, 6:54 AM KINSC Scopes Hoang
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Follow instructions for Making Measurements Using ImageJ in the Protocols section  5626k v. 5 Jun 25, 2012, 6:54 AM KINSC Scopes Hoang
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Follow instructions for Making Measurements Using ImageJ in the Protocols section  5626k v. 4 Jun 25, 2012, 6:54 AM KINSC Scopes Hoang
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Follow instructions for Making Measurements Using ImageJ in the Protocols section  5626k v. 4 Jun 25, 2012, 6:54 AM KINSC Scopes Hoang
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Protocol for Fluorescence and Brightfield Microscopy and includes taking pictures.  854k v. 3 Mar 13, 2012, 12:07 PM KINSC Scopes Hoang
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How to calibrate ImageJ with known table of values. The Table of Known Distances can only be used for the IMF and Confocal scopes using the Photometrics Coolsnap Cameras.  1394k v. 3 Mar 13, 2012, 12:13 PM KINSC Scopes Hoang
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Shows you how to calibrate ImageJ using images of a stage micrometer taken with the scope you are using. You may use this method to calibrate ImageJ for every scope that you use.  1191k v. 6 Mar 13, 2012, 12:16 PM KINSC Scopes Hoang
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