Primary Literature



Martinez, M. G. and Kielian, M. (2016) "Intercellular Extensions Are Induced by the Alphavirus Structural Proteins and Mediate Virus Transmission." PLoS Pathog 12(12): e1006061. 

Dubé, M.Etienne, L.Fels, M., and Kielian, M. (2016) "Calcium-dependent Rubella virus fusion occurs in early endosomes." J Virol 90(14):6303-13.

Stiles, K. M. and Kielian, M. (2016) "Role of TSPAN9 in alphavirus entry and early endosomes." J Virol 90(9):4289-97. [Selected for highlight by editors.] 


Fields, W. and Kielian, M. (2015) "Interactions involved in pH protection of the alphavirus fusion protein." Virology 486:173-9.

Ooi, Y.S., Dubé, M., and Kielian, M. (2015) "BST2/Tetherin inhibition of alphavirus exit." Viruses 7(4):2147-67.

Dubé, M., Rey, F.A., and Kielian, M. (2014) "Rubella virus: first calcium-requiring viral fusion protein." PLoS Pathog. 10(12): e1004530.

Zheng, A., Yuan, F., Kleinfelter, L.M., and Kielian, M. (2014) "A toggle switch controls the low pH-triggered rearrangement and maturation of the dengue virus envelope proteins.Nat. Commun. 5:3877.

Martinez, M.G., Snapp, E.L., Perumal, G.S.
, Macaluso, F.P., and Kielian, M. (2014) "Imaging the Alphavirus Exit Pathway.J. Virol. 88(12):6922-33. [Selected for cover.] 


Ooi, Y.S.*, Stiles, K.M.*, Liu, C.Y.*, Taylor, G.M., and Kielian, M. (2013) "Genome-wide RNAi screen identifies novel host proteins required for alphavirus entry.." PLoS Pathog. 9(12): e1003835. *These authors contributed equally to this work.

Uchime, O.*, Fields, W.*, and Kielian, M. (2013) "The role of E3 in pH protection during alphavirus assembly and exit." J. Virol. 87(18):10255-62. *These authors contributed equally to this work.
Zheng, Y. and Kielian, M. (2013) "Imaging of the alphavirus capsid protein during virus replication." J. Virol. 87(17):9579-89.

Sánchez-San Martín, C.,
Nanda, S., Zheng, Y., Fields, W., and Kielian, M. (2013) "Cross-inhibition of chikungunya virus fusion and infection by alphavirus E1 domain III proteins.J. Virol. 87(13):7680-7.

Fields, W. and Kielian, M. (2013) "
A Key interaction between the alphavirus envelope proteins responsible for initial dimer dissociation during fusion." J. Virol. 87(7):3774-81.



Roman-Sosa, G. and Kielian, M. (2011) "The interaction of alphavirus E1 protein with exogenous domain III defines stages in virus-membrane fusion." J. Virol. 85(23):12271-9

Zheng, Y., Sánchez-San Martín, C., Qin, Z., and Kielian, M. (2011) "The Domain I-Domain III linker plays an important role in the fusogenic conformational change of the alphavirus membrane fusion protein." J. Virol. 85(13):6334-42[Selected for highlight by editors.] 

Zheng, A., Umashankar, M., and Kielian, M. (2010) "In vitro and in vivo studies identify important features of dengue virus pr-E protein interactions." PLoS Pathog. 6(10):e1001157

Liu, C.Y.,
Besanceney, C., Song, Y., and Kielian, M. (2010) "Pseudorevertants of Semliki forest virus fusion-block mutation reveal a critical interchain interaction in the core trimer." J. Virol. 84(22):11624-33. 

Liao, M., 
Sánchez-San Martín, C., Zheng, A., and Kielian, M. (2010) "In vitro reconstitution reveals key intermediate states of trimer formation by the dengue virus membrane fusion protein." J. Virol. 84(11):5730-40


Sánchez-San Martín, C., Sosa, H., and Kielian, M. (2008) "A stable prefusion intermediate of the alphavirus fusion protein reveals critical features of class II membrane fusion." Cell Host Microbe 4(6):600-8. 

Umashankar, M., Sánchez San Martín, C., Liao, M., Reilly, B., Guo, A., Taylor, G., and Kielian, M. (2008) "Differential cholesterol binding by class II fusion proteins determines membrane fusion properties." J. Virol. 82(18):9245-53. 


Taylor, G.M., Hanson, P.I., and Kielian, M. (2007). "Ubiquitin depletion and dominant-negative VPS4 inhibit rhabdovirus budding without affecting alphavirus budding." J. Virol. 81(24):13631-9. 

Markosyan, R.M., Kielian, M., and Cohen, F.S. (2007) "Fusion induced by a class II viral fusion protein, semliki forest virus E1, is dependent on the voltage of the target cell.J. Virol. 81(20):11218-25.


Liao, M. and Kielian, M. (2005) “Domain III from class II fusion proteins functions as a dominant-negative inhibitor of virus-membrane fusion.J. Cell Biol. 171(1):111-20. 


Gibbons, D.L., Reilly, B., Ahn, A., Vaney, M.C., Vigouroux, A., Rey, F.A., and Kielian, M. (2004) "Purification and crystallization reveal two types of interactions of the fusion protein homotrimer of Semliki Forest virus.J. Virol. 78(7):3514-23.

Gibbons, D.L., Ahn, A., Liao, M., Hammar, L., Cheng, R.H., and Kielian, M. (2004) "Multistep regulation of membrane insertion of the fusion peptide of Semliki Forest virus.J. Virol. 78(7):3312-8.

Gibbons, D.L., Vaney, M.C., Roussel, A., Vigouroux, A., Reilly, B., Lepault, J., Kielian, M., and Rey, F.A. (2004) “Conformational change and protein-protein interactions of the fusion protein of Semliki Forest virus.” Nature 427(6972): 320-5.  


Gibbons, D.L., Erk, I., Reilly, B., Navaza, J., Kielian, M., Rey, F.A., and Lepault, J. (2003) "Visualization of the target-membrane-inserted fusion protein of Semliki Forest virus by combined electron microscopy and crystallography.Cell 114(5):573-83.

Zhang, X., Fugère, M., Day, R., and Kielian, M. (2003) "Furin processing and proteolytic activation of Semliki Forest virus.J. Virol. 77(5):2981-9.


Chatterjee, P.K., Eng, C.H., and Kielian, M. (2002) "Novel mutations that control the sphingolipid and cholesterol dependence of the Semliki Forest virus fusion protein.J. Virol. 76(24):12712-22.

Samsonov, A.V., Chatterjee, P.K., Razinkov, V.I., Eng, C.H., Kielian, M., and Cohen, F.S. (2002) "Effects of membrane potential and sphingolipid structures on fusion of Semliki Forest virus.J. Virol. 76(24):12691-702.

Ahn, A., Gibbons, D.L., and Kielian, M. (2002) "The fusion peptide of Semliki Forest virus associates with sterol-rich membrane domains.J. Virol. 76(7):3267-75.


Lu, Y.E., Eng, C.H., Shome, S.G., and Kielian, M. (2001) "In vivo generation and characterization of a soluble form of the Semliki forest virus fusion protein.J. Virol. 75(17):8329-39.


Gibbons, D.L., Ahn, A., Chatterjee, P.K., and Kielian, M. (2000) " Formation and characterization of the trimeric form of the fusion protein of Semliki Forest Virus.J. Virol. 74(17):7772-80.

Lu, Y.E. and Kielian, M. (2000). "Semliki forest virus budding: assay, mechanisms, and cholesterol requirement.J. Virol. 74(17):7708-19.

Kielian, M., Chatterjee, P.K., Gibbons, D.L., and Lu, Y.E. (2000). "Specific roles for lipids in virus fusion and exit. Examples from the alphaviruses.Subcell. Biochem. 34:409-55.

Chatterjee, P.K., Vashishtha, M., and Kielian, M. (2000). "Biochemical consequences of a mutation that controls the cholesterol dependence of Semliki Forest virus fusion.J. Virol. 74(4):1623-31.



Kielian, M. (2014) "Mechanisms of virus membrane fusion proteins." Annu. Rev. Virol. 1:171-89.

rson, T.C. and Kielian, M. (2013) "Flaviviruses: braking the entering." Curr. Opin. Virol. 3(1):3-12.

Kielian, M., Chanel-Vos, C., and Liao, M. (2010) “Alphavirus entry and membrane fusion.” Viruses 2(4):796-825.

Sánchez-San Martín, C., Liu, C.Y., and Kielian, M. (2009) "Dealing with low pH: entry and exit of alphaviruses and flaviviruses." Trends Microbiol. 17(11):514-21. 

Kielian, M. (2006) “Class II virus membrane fusion proteins.” Virology 344(1):38-47.  

Kielian, M. and Rey, F.A. (2006) “Virus membrane fusion proteins: more than one way to make a hairpin.” Nat. Rev. Microbiol. 4(1):67-76.  


Kielian, M. and E. Ollman Saphire. (2015) “Potent antibody protection against an emerging alphavirus threat.” Cell 163:1053-1054.

Stiles, K.M. and Kielian, M. (2011) "Alphavirus entry: NRAMP leads the way.Cell Host Microbe 10(2):92-3.

Kielian, M. (2010) “Structural biology: An alphavirus puzzle solved.” Nature 468(7324):645-6.  

Kielian, M. (2002) "Structural surprises from the flaviviruses and alphaviruses.Mol. Cell. 9(3):454-6.


Frei, J.C., Kielian, M., and Lai, J.R. (2015) "Comprehensive mapping of functional epitopes on dengue virus glycoprotein E DIII for binding to broadly neutralizing antibodies 4E11 and 4E5A by phage display." Virology 485:371-82.

Kleinfelter, L.M., Jangra, R.K., Jae, L.T., Herbert, A.S., Mittler, E., Stiles, K.M., Wirchnianski, A.S., Kielian, M., Brummelkamp, T.R., Dye, J.M., and Chandran, K. (2015) "Haploid genetic screen reveals a profound and direct dependence on cholesterol for hantavirus membrane Fusion.mBio 6(4):e00801-15.

Vigant, F., Lee, J., Hollmann, A., Tanner, L.B., Akyol Ataman, Z., Yun, T., Shui, G., Aguilar, H.C., Zhang, D., Meriwether, D., Roman-Sosa, G., Robinson, L.R., Juelich, T.L., Buczkowski, H., Chou, S., Castanho, M.A., Wolf, M.C., Smith, J.K., Banyard, A., Kielian, M., Reddy, S., Wenk, M.R., Selke, M., Santos, N.C., Freiberg, A.N., Jung, M.E., and Lee, B. (2013) "A mechanistic paradigm for broad-spectrum antivirals that target virus-cell fusion.PLoS Pathog. 9(4):e1003297.

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