1. Cellular Biomechanics
We are interested in mechanistically how biological cells "know" and respond to externally applied mechanical forces. We are developing state-of-art experimental models and devices and exploring the mechanosensing dynamics of red blood cells, circulating cancer cells and primary erythroid cells. We expect the outcomes of our investigation will fundamentally advance our understanding of mechanobiology and enhance our
ability to treat diseases with effective therapeutic strategies.
Wei, H., Kang, H., Rasheed, I-Y., Luo, N., Zhou, S., Wang, Y., Richardson,
K., Palmer, A., Xu, C., Wan, J.,
Nedergaard, M. (2016) Erythrocytes are oxygen-sensing regulators of the
cerebral microcirculation. Neuron, in press.
Fan, R., Emery, T., Zhang, Y., Xia, Y., Sun, J., Wan, J. (2016) Effect of circulatory
shear flow on the viability and proliferation of circulating colon cancer
cells. Sci. Rep. 6, 27073.
Fan, R., Emery, T., Zhang, Y., Xia, Y., Sun, J., Wan, J. (2016) In vitro microfluidic
circulatory system for circulating cancer cells. Nature Protocol
Cinar, E., Zhou S., DeCourcey, J., Wang, Y., Waugh,
R.E., Wan, J. (2015) Piezo1
regulates mechanotransductive release of ATP from human red blood cells. Proc.
Nat. Acad. Sci. USA, 112,
Forsyth A. M., Wan, J., Owrutsky, P.D.,
Abkarian, M., and Stone, H. A. (2011) A multiscale approach to link red blood
cell dynamics, shear viscosity, and ATP release. Proc. Nat. Acad. Sci. USA, 108, 10986-10991.
Wan, J., Forsyth, A. M., and Stone, H. A. (2011)
Red blood cell dynamics: from cell deformation to adenosine-5'-triphosphate
release. Integr. Biol. 3,
Wan, J., Ristenpart, W. D., and Stone, H. A.
(2008) Dynamics of shear-induced ATP release from red blood cells. Proc.
Nat. Acad. Sci. USA. 105,
We demonstrate a microfluidic approach to investigate the dynamics of shear-triggered release of ATP from human red blood cells. (A) Schematic of the experimental apparatus (not to scale). A mixture of RBCs and luciferase/luciferin solution are pumped through a microfluidic constriction. (B) Representative experimental measurements of the photon emission rate resulting from the reaction between luciferase/luciferin and ATP, measured versus position along the channel.
2. Microvascular and Tissue Engineering
of a vascular architecture capable of distributing oxygen and nutrients within
engineered tissue constructs is critically important for the development of functional
living organs. In particular, controlled
engineering of complex, hierarchical microvascular networks remains a
challenging yet unaccomplished task. Although formations of microvascular
architectures using methods such as direct writing, microfluidics, and electrical discharge have been demonstrated, effective
approaches to produce functional three-dimensional (3D) complex microvascular
networks are still missing. Lack of such a system is an important problem,
because, without it, acquiring the ability to sustain 3D tissue culture constructs
with a physiologically relevant size is highly unlikely, and consequently in vitro development of transplantable organ
constructs will be extremely difficult.
We aim to develop novel technologies and materials to construct 3D microvascular networks and thus to engineer vascularized tissue constructs. Such results are expected to have an important positive impact, because in
addition to provide novel and innovative technology for tissue engineering, the developed approaches are highly
likely to provide functional microvascular grafts and transplantable tissues for clinical
Piou, M., Fan, R., Darling, E., Cormier, D., Sun, J., Wan. J. (2016) Bioprinting cell-laden Matrigel/agarose constructs. Annals Biomedical Engineering. in review.
Fan, R., Naqvi, K., Patel, K., Sun, J., Wan, J. (2015) Microfluidic generation of oil-free cell-containing hydrogel particles. Biomicrofluidics. 9, 052602.
Fan, R., Sun, Y., Wan, J. (2015) Leaf-inspired artificial microvascular networks (LIAMN) for 3D cell culture. RSC Advances. 5, 90596-9060
We demonstrate a concept of leaf-inspired artificial microvascular networks (LIAMN) for 3D cell culture in hydrogel constructs. A framework of branching models was incorporated to construct LIAMN that enables a long-term cell culture and the formation of spheroid in 3D hydrogel. The presented approach is expected to pave a new way to realize vascularization in 3D cell culture and the development of functional artificial organs. We are also developing 3D printing technologies for tissue engineering.
3. Complex fluids, emulsions, and functional materials
We aim to provide fundamental understandings of fluid behavior at small scales and particularly the roles of microfluidics in emulsions (droplets and bubbles), chemical reactions and material synthesis. We are currently developing microfluidic approaches and/or 3D printing techniques to investigate 1) bio-inspired materials and technology for drug delivery and imaging, and 2) energy storage materials and solar fuels.
| Representative Publications
Lu, T., Fan, R., Delgadillo, L., Wan, J. (2016) Stabilization of carbon dioxide (CO2) bubbles in
micrometer-diameter aqueous droplets and the formation of hollow
microparticles. Lab On Chip. 16, 1587-1592. (Featured as cover
Koppula, K. S., Veerapalli, K. R., Fan, R., Wan, J. (2016) Integrated microfluidic
system with simultaneous emulsion generation and concentration. J Colloid Interface Sci. 466, 162-167.
Ge, H. Xu, H., Lu, T., Li, J., Chen, H., Wan, J. (2015) Microfluidic production
of porous carbon spheres with tunable size and pores. J Colloid Interface Sci. 461, 168-172.
Li, J., Wang,
Y., Chen, H., Wan, J. (2014) Electrowetting on dielectrics for
manipulating oil drops and gas bubbles in aqueous-shell compound drops. Lab on Chip. 14, 4334-4337.
Shim, S., Wan,
J., Hilgenfeldt, S., Panchal, P., Stone, H. A. (2014) Dissolution without disappearing:
multicomponent gas exchange for CO2 bubbles in a microfluidic channel. Lab on Chip. 14, 2428–2436.
Chen, H., Li, J., Wan, J., Weitz, D. A., and
Stone, H. A. (2013) Gas-core
triple emulsions for ultrasound triggered release. Soft Matter. 9, 38-42. (Featured as back cover article).
Shi, L., Benson, B., Bruzek, M. J., Anthony, J. E., Sinko, P. J., Prudhomme, R.
K., and Stone H. A. (2012) Microfluidic generation of droplets with a
high-loading of nanoparticles. Langmuir. 28, 13143–13148.
(2012) Microfluidic-based synthesis of hydrogel particles for cell
microencapsulation and cell-based drug delivery. Polymers, 4, 1084-1108. Invited Review.
We are developing microfluidic approaches to synthesis functional particles and nanomaterials for drug delivery, energy storage and photo catalytic CO2 reduction.