Lab protocols

Protocols for protein production and purification for NMR experiments. 

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More online protocols

These are some laboratory protocols with which I have had great success. 

General

 primer design

cloning

agarose gels 

transformation

SDS-PAGE

UV-VIS - links to U. Wis/Mads website that explains it well. Our procedure.

Chromatography columns (information/procedures) 

 
The Bicoid Homeodomain

plasmid construction

expression

purification

making a protein/DNA NMR sample 

troubleshooting 

The TEV Protease

general information 

expression

purification