Welcome to Dr. Jagadis Gupta Kapuganti Research Page

  
 
 

Dr JAGADIS GUPTA KAPUGANTI


DEPARTMENT OF PLANT SCIENCES

UNIVERSITY OF OXFORD

SOUTH PARKS ROAD

OXFORD, OX1 3RB

UNITED KINGDOM

EMAIL: jagadis.kapuganti@plants.ox.ac.uk

 
Degree

MSc: University of Hyderabad, India, 2003

PhD: University of Wuerzburg, Germany, 2007

 

 
                                                                      

Hindrattan

 
 
 
 
 

 
 
 
 
 

AREAS OF INTEREST AND EXPERTISE

 

NITRIC OXIDE SOURCES AND SIGNALING REACTIVE OXYGEN SPECIES,  ENERGY METABOLISM, PHYSIOLOGY OF ALTERNATIVE OXIDASE REGULATION, LOW OXYGEN SENSING,

NITROGEN METABOLISM NITROGEN CYCLES AND IMPACTS ON PLANT METABOLISM

PLANT PATHOGEN AND PLANT SYMBIOTIC INTERACTIONS,

PHOTORESPIRATORY SERINE HYDROXY METHYLTANSFERASES (SHMTS)

 

 

 

 

 

 
          
 
 
 
  
 
 
 
 
RESEARCH EMPHASIS
 

Nitric Oxide sources and signalling in hypoxic stress

 

In analogy to the multiple functions of NO in animals, during the past decade a similar scenario has been developed in plant science. Plants have probably several enzyme systems to produce NO. Potential candidates are: assimilatory nitrate reductase (NR), xanthine dehydrogenase (XDH), a PM-nitrate reductase (PM-NR) plus an associated nitrite:NO reductase, NO-synthase (NOS), and eventually a reaction associated with mitochondrial electron transport chain of roots and cell suspensions.In additional to this non sysmbiotic haemoglobin scavenge nitric oxide I am currently working on production and scavenging pathways of NO production  and its role in hypoxia. we recently discovered that plant mitochondria are able to produce ATP under anoxic conditions by using nitrite as a terminal electron acceptor. We also discovered new pathway of NO production which uses hydroxylamine as a substrate.

 
 
 
 
 
 
 
 
 
The figure illustrates operation of root mitochondrial electron transport chain under hypoxic conditions. Plant mitochondria can produce limited amount of ATP under anoxic conditions where nitrite acts as electron acceptor   

 
 

 

 

 

Figure shows structure of pentacoordinate and hexacoordinate hemoglobins. Coordination of proximal (HP) and distal (HD) histidines in pentacoordinate and hexacoordinate heme. The pentacoordinate structure is open for reversible binding of ligands such as O2 and NO, while the hexacoordinate structure facilitates tight binding of oxygen that can further accept an electron from iron and oxygenate NO resulting in formation of nitrate (NO3-).

 
 
Photorespiration
 
Serine hydroxymethyltransferases (SHMs) are important enzymes present in all eukaryotes. SHMTs catalyze the interconversion of serine to glycine, whereby one-carbon building blocks for biosynthetic purposes are produced.
In Arabidopsis genome SHMTs are endoced by multigenefamily. Arabidopsis Serine hydroxyl methyl transferase 6 AtSHM6 protein contains 599 amino acids, and it is encoded by At1g22020. In silico analysis indicated that this protein is present in nucleus. Currently I am investigating the role of SHMTs in various biological processes associated with photorespiratory metabolism.

 


Structure of AtSHM6 

 
 

 

 

  

Role of nitrogen nutrition on plant pathogen Interactions:

 

 

 

 

 

Plants need nitrogen because it’s a major constituent of the plants. Nitrate and ammonium are the major N sources for the plants. Biological mineralization provides nitrate or ammonium to the plants. In agriculture soils nitrogen is supplied in the form of nitrate, ammonium or combination of both. Ammonium as a N form of nitrogen enters into the soil via fixation of atmosphere nitrogen; decomposition of organic matter by bacteria and fungi, By nitrification pathway ammonium is oxidized to nitrite and further nitrate. Nitrate is a component of many primary and secondary metabolites In this project I am investigating effect of N nutrition on plant resistance to pathogens. I am using Tobacco and Pseudomonas syringie pv phaseolicola as model systems for the investigation.
 
 
Publications in high impact factor Journals
 
    IF  13.4         IF 6.8              IF 9.8          IF 7.05             IF 7.9
 
 
   IF 4.6               IF 4.1              IF  9.9             IF 6.5              IF 5.7

 

RESEARCH IS FUNDED BY


 

 

 COLLABORATIONS

 

Prof Werner Kaiser University of Wuerzburg, Germany

 Dr Andrei Igamberdev, Memorial University, Canada

Prof Franziska Krajinski , MPIMP, Golm

 Dr Luis  Mur, Aberystwyth Universtiy, Wales, UK

Dr Alisdair Fernie, MPIMP, Golm

Dr Jose F Moran, Agriculture Biotechnology Institute, Navarra Spain

Dr Kim Hebelstrup Genetics and Biotechnology Research Centre, Aarhus, Denmak

Dr Yariv Brotman, MPI, Golm
 
                                       
 
 
 
 Editorial activities:

From 2013 Editorial review  board member: Journal of Experimental Botany

Associate Editor and Member of editorial board: From 2009 till date  Plant Signaling and Behaviour

From 2009 Editorial board member IIOAB journal
 

 
Reviewer
 

Plant Cell (2010)

The Plant Journal (2012;2013)

Journal of Experimental Botany (2009, 2010, 2011, 2012, 2013)

Plant Cell & Environment (2010, 2011. 2012, 2013)

Plant Biology (2010)

Environmental and Experimental Botany (2011)

Physiologia Plantarum (2013)

BMC Plant Biology (2012)

FEBS Letters (2011)

New Phytologist (2010, 2011, 2012, 2013, 2014)

Plant Signalling & Behaviour (2012)

Tree Physiology (2011)

Plant Physiology & Biochemistry (2013)

Annals of Botany (2013)

Planta (2013)

 

 

 Highlights

 

 

 
 

Trichoderma mycoparasitism.

(A) Mycoparasitic confrontation assay and (B) coiling of Trichoderma (T) hyphae around the plant pathogen Rhizoctonia solani (R).
 

 Brotman Y, Gupta KJ, Vitrebo A: Current Biology 2010

 
 
 

News

Evaluated articles in Faculty of 1OOO Biology

 Zabalza A, van Dongen JT, Froehlich A, Oliver S, Faix B, Gupta KJ, Schmalzlin E, Igal M, Orcaray L, Royuela M, Geigenberger P (2009) Regulation of respiration and fermentation to control plant internal oxygen concentration
 
Stoimenova M, Igamberdev AU, Gupta KJ, Hill RD (2007) Nitrite driven anaerobic ATP synthesis by barley and rice root mitochondria. Planta 226: 465-474
 
 
 
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          JAGADIS NAME IS INCLUDED IN MARQUIS WHO'S WHO WORLD 2013
 
 
  
Oxford Research Staff Society (OxRSS)
 
JAGADIS IS 2012-2014 MPLS divisional coordinator of Oxford Research Staff Society


 

 

 

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