Publications & Patents

(Issadore Lab members in bold, #: equal contribution)


Journal Articles 

2017

(45) H. Jeong, S.H. Han, S. Yadavali, J. Kim, D. Issadore#, D. Lee#, Moldable Perfluoropolyether-Polyethylene Glycol Networks with Tunable Wettability and Solvent Resistance for Rapid Prototyping of Droplet Microfluidics, 2017 (submitted).

(44) J. Ko, N. Bhagwat, T. Black, S. Yee, Y. Na, S. Fisher, J. Kim, E. Carpenter, B. Stanger, and D. Issadore, Development of an Extracellular Vesicle microRNA Biomarker Panel for Machine Learning Based Diagnosis of Pancreatic Cancer, 2017 (submitted).

(43) J.E. Mealy, J.J. Chung, H. Jeong, D. Issadore, D. Lee, P. Atluri, J.A. Burdick, Self-assembled and injectable colloidal hydrogels with multifunctional properties for biomedical applications, 2017 (submitted).

(42) S. YadavaliH. Jeong, D. Lee, D. IssadoreAll Silicon and Glass, Very Large Scale Microfluidic Droplet Integration for the Terascale Generation of Polymer Microparticles, 2017 (submitted).

(41) J. Ko, M. Hemphill, E. Sewell, YJ. Na, D. K. Sandsmark, S. A. Fisher, E. A. Torre, R. Diaz-Arrastia, J. Kim, D. F. Meaney, D. IssadorePrognosis of traumatic brain injury using machine learning based miRNA signatures in nanomagnetically isolated brain-derived exosomes, 2017 (submitted).

(40) J. Ko#, S. N. Baldassano#, P. Loh, K. Kording, B. Litt, D. Issadore,  Machine Learning To Detect Signatures of Disease in Liquid Biopsies - A User’s Guide, Lab on a Chip, DOI: 10.1039/C7LC00955K, 2017.


(39) J. Ko, N. Bhagwat, S. S. Yee, N. Ortiz, A. Sahmoud, T. Black, N. M. Aiello, L. McKenzie, M. O’Hara, C. Redlinger, J. Romeo, E. L. Carpenter, B. Z. Stanger, D. Issadore, Combining Machine Learning and Nanofluidic Technology to Diagnose Pancreatic Cancer Using Exosomes, ACS NanoDOI: 10.1021/acsnano.7b05503, 2017.


(38) J. F. LiuS. Yadavali, A. Tsourkas#, David Issadore#, Microfluidic Diafiltration Using an Integrated Magnetic Peristaltic Micro-Pump, Lab on a Chip, DOI:10.1039/C7LC00954B, 2017.


(37) J. Ko, N. Bhagwat, S. S. Yee, C. Redlinger, J. Romeo, M. O’Hara, A. Raj, E. L. Carpenter, B. Z. Stanger, D. IssadoreA magnetic micropore chip for rapid (< 1 hour) unbiased circulating tumor cell isolation and in-situ RNA analysis, Lab on a Chip, DOI: 10.1039/C7LC00703E, 2017.


(36)  H. Jeong, S. YadavaliD. Issadore#, D. Lee#, Liter-scale Production of Uniform Gas Bubbles via Parallelization of Flow Focusing Generators,  Lab on a Chip, DOI: 10.1039/C7LC00295E, 2017.


(35) VR. Yelleswarapu, H. Jeong, S. Yadavali, D. IssadoreUltra-High Throughput Detection (1 Million Droplets / Second) of Fluorescent Droplets Using a Cell Phone Camera and Time Domain Encoded Optofluidics, Lab on a Chip, DOI: 10.1039/C6LC01489E, 2017.


   Animation of how the device works:




2016

(34) J. Ko, M. A. Hemphill, D. Gabrieli, L. Wu, VR. Yelleswarapu, G. Lawrence, W. Pennycooke, A. Singh, D. Meaney, D. IssadoreSmartphone-enabled optofluidic exosome diagnostic for concussion recovery, Scientific Reports, 631215, 2016.

(33) J. Ko#, V.R. Yelleswarapu#, A. Singh, N. Shah, D. IssadoreMagnetic Nickel iron Electroformed Trap (MagNET): A master / replica fabrication strategy for ultra-high throughput (> 100 mL/hr) immunomagnetic sorting, Lab on a ChipDOI: 10.1039/C6LC00487C, 2016.

(32) M. Magaraci, J. Bermudez,  D. Yogish, D. Pak, V. Mollov, J. Tycko, D. Issadore, S. Mannickarottu,B. Chow, Toolbox for Exploring Modular Gene Regulation in Synthetic Biology Training, ACS Synthetic BiologyDOI: 10.1021/acssynbio.6b00057,  2016.

(31) H. Jeong, D. Issadore, D. Lee. Recent Developments in Scale-up of Microfluidic Emulsion Generation via Parallelization, The Korean Journal of Chemical Engineering, DOI: 10.1007/s11814-016-0041-6, 2016.

(30) J. Ko, E. Carpenter*, D. Issadore*Detection and isolation of circulating exosomes and microvesicles for cancer monitoring and diagnostics using micro- /nano- based devices, AnalystDOI: 10.1039/C5AN01610J, 2016.

2015

(29) H. Shao, J. Chung, D. IssadoreDiagnostic Technologies for Circulating tumor cells and Exosomes, Bioscience ReportsDOI: 10.1042/BSR20150180, 2015.

(28) H.H. Jeong, V. R. YelleswarapuS. YadavaliD. Issadore*, D. Lee*, Kilo-scale droplet generation in three-dimensional monolithic elastomer device (3D MED), Lab on a Chip, DOI: 10.1039/C5LC01025J, 2015. (highlighted as a Hot Article 2015)




(27) S. Shaffer, R. Joshi, B. Chambers, D. Sterken, A. Biaesch, D. Gabrieli, Y. Li, K. Feemster, S. Hensley*, D. Issadore*, A. Raj*, Multiplexed detection of viral infections using rapid in-situ RNA analysis on a chip, Lab on a Chip, DOI: 10.1039/C5LC00459D, 2015.


(26) D. IssadorePoint-of-care rare cell cancer diagnostics, Methods in Molecular Biology1256, 123-137, 2015.


2014

    (25) M. Muluneh, B. Kim, G. Buchsbaum, D. IssadoreMiniaturized, multiplexed readout of droplet-based microfluidic assays using time-domain modulation, Lab on a Chip14, 4638-4646, 2014. (highlighted as a Hot Article of 2014)

    (24) M. MulunehD. IssadoreA multi-scale PDMS fabrication strategy to bridge the size mismatch between integrated circuits and microfluidics, Lab on a Chip14, 4552-4558, 2014.


    (23) D. IssadoreY. I. Park, H. Shao, C. Min, K. Lee, M. Liong, R. Weissleder, H. Lee, Magnetic sensing technology for molecular analyses, Lab on a Chip, 10.1039/C4LC00314D, 2014.


    (22) M. Muluneh, W. Shang, D. Issadore, Track‐Etched Magnetic Micropores for Immunomagnetic Isolation of Pathogens. Advanced Healthcare MaterialsDOI: 10.1002/adhm.201300502, 2014. This work was featured on the cover.
                

    (21) C. Castro, A. Ghazani, J. Chung,  H. Shao, D. Issadore, TJ Yoon, R. Weissleder, H. Lee, Miniaturized Nuclear Magnetic Resonance Platform for Detection and Profiling of Circulating Tumor Cells. Lab on a Chip, DOI: 10.1039/C3LC50621E, 2014.
                

    (20) M. MulunehD. Issadore, Microchip-based detection of magnetically labeled cancer biomarkers. (Invited Review) Advanced Drug Delivery Reviews, 66, 101-109, 2014.
                
                


    2013

    (19) B. Chumo, M. Muluneh, D. Issadore, "Laser Micromachined Hybrid Open / Paper Microfluidic Chips." Biomicrofluidics7, 064109,  2013.
                
            


    (18) M. Muluneh, D. Issadore, "Hybrid soft-lithography / laser machined microchips for the parallel generation of droplets." Lab on a ChipDOI:10.1039/C3LC50979F, 2013. (youtube)
                
                

    The Droplet Factory


      (17) J. Chung, D. Issadore, A. Ullal, R. Weissleder, H. Lee, "Rare cell detection and profiling on a hybrid magnetic / size-sorting (HMSS) chip." Biomicrofluidics, DOI:10.1063/1.4821923, 2013.
                  

      (16) D. Koziej, C. Floryan, R. A. Sperling, A. Ehrlicher, D. Issadore, R. Westervelt, and D.A. Weitz, "Microwave dielectric heating of non-aqueous droplets in a microfluidic device for nanoparticles synthesis." Nanoscale, DOI: 10.1039/c3nr00500c, 2013. (featured in SPIE newsroom)
                  

      (15) D. Issadore, H. Chung, G. Budin, R. Weissleder, H. Lee, "µHall chip for sensitive detection of bacteria." Advanced Healthcare Materials, DOI: 10.1002/adhm.201200380, 2013.
                  

      2012

      (14) D. Issadore, J. Chung, H. Shao, R. Weissleder, H. Lee, “Ultrasensitive clinical enumeration of rare cells ex vivo using a µ-Hall detector.” Science Translational Medicine, 4, 141ra92, 2012. This work was featured on the cover
                  


      (13) J. Chung, H. Shao, T. Reiner, D. Issadore, R. Weissleder, H. Lee, “Microfluidic cell sorter (μFCS) for on-chip capture and analysis of single cells.”
      Advanced Healthcare Materials, 1, 365, 2012. This work was featured on the cover
                  

      (12) H. Shao, C. Min, D. Issadore, M. Liong, T.J. Yoon, R. Weissleder, H. Lee, “Magnetic nanoparticles and micro NMR for diagnostic applications.” Theranostics, 2, 55, 2012.


      2011

      (11) M.. Liong, M. Fernandez-Suarez, D. Issadore, C. Min, C. Tassa, T. Reiner, S. Fortune, M. Toner, H. Lee, R. Weissleder, “Specific pathogen detection using bioorthogonal chemistry and diagnostic magnetic resonance.” Bioconjugate Chemistry, 22, 2390, 2011.

                  
      (10) H. Chung , T. Reiner , G. Budin , C. Min , M. Liong , D. Issadore , H. Lee , and R. Weissleder, “Ubiquitous detection of gram-positive bacteria with bioorthogonal magnetofluorescent nanoparticles.” America Chemical Society Nano, 5, 8834, 2011.
                  

      (9) A.V. Ullal, T. Reiner, K.S. Yang, R. Gorbatov, C.Min, D. Issadore, H. Lee, R. Weissleder, “Nanoparticle mediated measurement of target-drug binding in cancer cells.” American Chemical Society Nano, 5, 9216, 2011.
                  
      (8) D. Issadore, C. Min, M. Liong, J. Chung, R. Weissleder, H. Lee, “Miniaturized diagnostic magnetic resonance platform for point-of-care medicine.” Lab on a Chip, 11, 2282, 2011. Amongst the top 10 most accessed articles.
                  
      (7) D. Issadore, H. Shao, J. Chung, A. Newton, M. Pittet, R. Weissleder, H. Lee, “Self-assembled magnetic filter for highly efficient immunomagnetic separation.” Lab on a Chip, 11, 147, 2011.
                  

      Before 2011

      (6) D. Issadore, T. Franke, K.A. Brown, R.M. Westervelt, “A microfluidic microprocessor: controlling biomimetic containers and cells using hybrid integrated circuit/microfluidic chips.” Lab on a Chip, 110, 2937, 2010.
                  


      (5)K. Brown, C.C. Vassiliou, D. Issadore, J. Berezovsky, M.J. Cima and R.M. Westervelt, “Scaling of transverse nuclear magnetic relaxation due to magnetic nanoparticle aggregation.”
      Journal of Magnetism and Magnetic Materials, 322, 3122, 2010.

      (4) D. Issadore, T. Franke, K.A. Brown, T.P. Hunt, R.M. Westervelt, “High voltage dielectrophoretic and magnetophoretic hybrid integrated circuit / microfluidic chip.” IEEE Journal ofMicroelectromechanical Systems, 6, 1220, 2009.
                  
      (3) D. Issadore, K.J. Humphry, K.A. Brown, L. Sandberg, D.A. Weitz, and R.M. Westervelt, “Microwave dielectric heating of drops in microfluidic devices.” Lab on a Chip, 12, 1701, 2009.
                  
      (2) T.P. Hunt, D. Issadore, and R.M. Westervelt, "Integrated circuit/ microfluidic chip to programmably trap and move cells and droplets with dielectrophoresis," Lab on a Chip, 8, 81, 2008. This work was featured on the cover 
                  

      DEP control of droplets


      (1) K.C. Ku, S.H. Chun, W.H. Wang, W. Fadgen, D. Issadore, N. Samarth, R.J. Epstein, and D.D. Awschalom, "Fabrication and characterization of modulation-doped ZnSe/(Zn,Cd)Se (110) quantum wells: a new system for spin coherence studies," Journal of Superconductivity, 18, 185, 2005.

      Books / Chapters in Books


      (4) C. Floryan, D. Issadore, and R. M. Westervelt, "Programmable Hybrid Integrated Circuit/Microfluidic Chips." Point-of-Care Diagnostics on a Chip. Springer Berlin Heidelberg, 23-43, 2013.

      (3) C. Min, H. Shao, D. Issadore, M. Liong, R. Weissleder, H. Lee, "Diagnostic Magnetic Resonance Technology." Point-of-Care Diagnostics on a Chip. Springer Berlin Heidelberg,197-222, 2013.

      (2) D. Issadore and R.M. Westervelt - Editors, Point of Care Diagnostics on a Chip. Springer, Berlin Heidelberg, 2013.

      (1) T.P. Hunt, D. Issadore, K. Brown, H. Lee, R.M. Westervelt, “Trapping and moving cells and droplets with dielectrophoresis.” Lab-on-a-chip technology: biomolecular separation and analysis, UK Horizon Scientific Press, 2009.


      Patents

      (11) D. Issadore and M. Muluneh, Track etched magnetic micropore filter for highly efficient immunomagnetic sorting. Patent Number: WO2015013364-A1, 2015.

      (10) A. Raj, D. Issadore, S. Schaffer, Methods for Rapid Ribonucleic Acid Fluorescence In Situ Hybridization, Patent Number: WO2015002978-A2. (Licensed by Biosearch), 2015.

      (9) D. Issadore, M. muluneh, A multi-scale PDMS fabrication strategy to bridge the size mismatch between integrated circuits and microfluidics, Provisional Patent Filed, 2014.

      (8) D. Issadore, M. Muluneh, Miniaturized, multiplexed readout of droplet-based microfluidic assays using time-domain modulation, Provisional Patent Filed, 2014.

      (7) D. Issadore, J. Ko, Apparatus and methods for making vesicles, Provisional Patent Filed, 2014.

      (6) D. Issadore and B. Chumo, Hybrid Open Channel - Paper channel microfluidic networks for instrumentation free portable medical diagnostics. Application filed, UOP-491USP, 2103.

      (5) R Weissleder, H Lee, and D Issadore, Analyte detection using magnetic Hall effect. Application filed, USSN 61/563,33, 2012.

      (4) R Weissleder, H Lee, and D Issadore, Detection of targets using magnetic resonance. WIPO Patent No. 2012129281, 2012.

      (3) R Weissleder, H Lee, and D. Issadore. Self-assembled magnetic arrays. WIPO Patent No. 2012047653, 2012.

      (2) T. Hunt, D. Issadore, and R.M., Method and apparatus for manipulation of fluid species. 60/947,063, 2008.

      (1) D. Issadore, T. Hunt, K. Adamson, R. Rogers, and R.M. Westervelt, Method and apparatus for near field irradiation. WO/2007/106402, 2007.


      * Equal contribution