The Journal of Veterinary Science

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Gunshot Injury in a Dog: A Case Report 

Kisani A.I.a*, Grema B. A.b, Wachida N.a, Okachi I.a and Adamu S.S.a 

a Department of Veterinary Surgery and Theriogenology, University of Agriculture, P.M.B 2373, Makurdi Benue State Nigeria 

b Veterinary Teaching Hospital, University of Agriculture, P.M.B. 2373, Makurdi Benue State Nigeria


Gunshot, Wounds, Second intention, pellets Corresponding Author: *Kisani A. I. Email: abohkisani (at) yahoo (dot) com 


A 2-year old sexually intact male dog was presented for treatment of gunshot wounds of 6-hours duration. Clinical examination revealed penetrating wounds on the head and neck. Fluid therapy (Lactated Ringers solution) was administered intravenously to help stabilised the patient. The wounds were carefully assessed and pellets lodged in the wounds were removed without surgical exploration because they were lodged in the subcutaneous tissue. The wounds were cleaned, dressed and allowed to heal by second intention. Broad spectrum antibiotic (amoxicillin) and analgesic (piroxicam) were administered to prevent secondary bacterial infection and pain. Healing was uneventful and the dog was discharged after one week of treatment.


Kisani A.I., Grema B.A., Wachida N., Okachi I. and Adamu S.S., 2018. Gunshot Injury in a Dog: A Case Report. The Journal of Veterinary Science. Photon 119, 504-510

All Rights Reserved with Photon. 

Photon Ignitor: ISJN17846372D876525042018

The study evaluated the effect of three protein levels in concentrate diets on weight gain, total serum glucose, protein levels and fertility in terms of conception, abortion, kidding, twining and kids survival. Treatment groups were high protein supplementation (28.24 %), medium protein supplementation (18.94 %) and low protein supplementation (10.3 %). Fifteen adult WAD does aged between 1-1½ years (body weight of 9-12 kg) were assigned into three treatment group of five each. Blood sample were collected twice weekly and serum was harvested throughout the experimental period to determine glucose and protein levels. Results of this study indicated that serum glucose level didn’t show any significant difference (p > 0.05) across the treatment groups. Serum protein levels showed significant differences (p < 0.05) only after CIDR withdrawal between high proteins (67.80 ± 1.48) and low protein (10.73 ± 0.58) and between medium protein (65.52 ± 1.50) and low protein (10.73 ± 0.58) but no significant difference (p > 0.05) was observed between high protein and medium protein levels. High protein levels favored kid’s survival, twining, conception, kidding and abortion rates. In conclusion, different level of protein intake affected reproductive performance of West African Dwarf does by lowering kid survival, twining, kidding, conception and abortion rate, 

The current trial was performed to investigate the influence of sunflower seeds on the oxidative status and incidence of FLHS in laying hens. Fifty (Hisex) bird, 20 week old, were obtained from Animal Production Research Center (kuku), were divided into two groups (n=25) for each one. The control group (A) was under control diet based on corn, maintains the (NRC, 1994) requirements for laying hens, the experimental group, supplemented by 10% sunflower seeds added to the diet. The trial lasted for eight weeks, blood samples were collected once per month (week 4 and 8), in EDTA coated vials, immediately placed into iced-container, centrifuged at 3000 rpm/20 min, samples were separated in aliquot, and stored at -20ºC and -80ºC until analysis. No incidence of fatty liver hemorrhagic syndrome was noticed after feeding laying hens diet containing 10% sunflower seeds for two months. High plasma concentrations of poly unsaturated fatty acids, and omega-6 fatty acids were recorded by group (B), the difference was significant at (p<0.05), compared to the control group (A). There was no difference in liver enzymes, MDA and vitamin C, levels between the control group and the treated one. While the treated group revealed significant plasma concentration of SOD and vitamin E compared to the control group. No FLHS histopathological changes were observed in the treated group. 

Infectious bursal disease (IBD) is a highly contagious viral disease of birds characterized by lesions in the bursa of Fabricius, immunosuppression, severe morbidity and mortality. The outbreaks of infectious bursal disease (IBD) have become a burning problem to the poultry industry worldwide. Here, we performed screening and genetic analysis of IBD virus (IBDV) field isolates from different poultry farms in Tirupati region of Andhra Pradesh. Due to amino acids substitutions in this region results in change in the topography of hyper variable region of VP2, where most of the neutralizing epitopes are present. Cloning and characterization of hyper variable region of prevalent strains of IBDV in this part of the country will provide basis for development of better vaccine for future. In the present study, we cloned hyper variable region of IBDV-VP2 and found that all the cloned sequences are more or less similar. Phylogenetic analysis showed that they are closely related to strains of Luxembourg, China, and USA. This study will enhance the knowledge on IBDV circulating strains in this part of the country.

Treatment of Bloat and Tympany in Bovines with Herbal Oral Emulsion 

S.K. Sahatpurea, K. Ravikanthb, Shivi Mainib and Ankush Reothiab* 

a Department of ARGO, Nagpur Veterinary College, Nagpur, India 

b Research & Development Division, Ayurvet Limited, Baddi, India 

Keywords: Bloat, Appetite, Ruminal fluid, Herbal, *Brand A; Bloatonil 


In present study the impact AV/ATB/35, herbal oral emulsion for tympany and bloat (Supplied by M/s Ayurvet Ltd, Baddi, HP; India) was evaluated against bloat condition in cows on the basis of improvement in clinical signs and rumen fluid analysis. The study was undertaken at the Nagpur Veterinary College, Maharashtra, India. 20 cows suffering from bloat were divided in two treatment groups T1 and T2 of 10 animals each. Group T1 animals were treated with AV/ATB/35 @50 ml bid for two consecutive days and Group T2 animals were treated with Brand A@50ml bid for two consecutive days. In group T1 abdominal distension was relieved within 1 hour as compared to 2 hours in group T2 animals. Appetite and ruminal functions were significantly improved in herbal oral emulsion supplemented Group T1 in comparison to slight improvement in Group T2 after treatment. Analysis of rumen fluid revealed that AV/ATB/35 restored ruminal pH, protozoal count and protozoal motility to normal more efficiently as compared to Brand A. AV/ATB/35, herbal oral emulsion was found to be effective to treat bloat cases of bovines in comparison to Brand A. AV/ATB/35 contains herbal extracts and carminative volatile oils which ensure quick and prompt relief in bloat cases hence may be successfully employed in the treatment of bloat. 


Sahatpurea S.K., Ravikanth K., Maini S. and Reothi A., 2016. Treatment of Bloat and Tympany in Bovines with Herbal Oral Emulsion. The Journal of Veterinary Science. Photon 117, 465- 467 All Rights Reserved with Photon. 

Photon Ignitor: ISJN17846372D835106062016

Dr. Gidda Maheswaruduis conferred withCharles Darwin Research Award-2015 in Zoology


Siddiqui M. F. M. F., Waghmare S. P., Mode S.G., Pajai K.S., Adarsh, Maini S., Ravikanth, K., 2014. A herbal tool- AV/KPC/10 to check the sub clinical ketosis in cows. The Journal of Veterinary Science. Photon 116, 457-464 


Ketosis is a metabolic disorder that occurs in cattle when energy demands exceed energy intake and result in a negative energy balance. The current study was designed to evaluate the efficacy of herbal gel AV/KPC/10 (M/S Ayurvet Limited) for control of sub clinical ketosis in cows. 18 animals positive for sub clinical ketosis were randomly divided into Group I (n=6), treated with AV/KPC/10 at dose 200 gm twice a day orally for first two days followed by 100 gm once a day for next 3 days, Group II (n=6), treated with Liquid Anabolite at dose 200ml twice a day orally for two days, followed by 100 ml twice a day for 3 days and Group III (n=6), treated with Inj. Dextrose 25% at dose 500 ml intravenously once in a day for three days. Total 12 healthy cows negative for sub clinical ketosis were selected and divided randomly into two equal groups, control Group IV (n=6), cows negative for sub clinical ketosis were kept without any treatment and treatment Group V (n=6), cows negative for sub clinical ketosis were supplemented with AV/KPC/10. Parameters viz. Ketosis diagnosis, biochemical estimation and milk yield were recorded before treatment (‘0’ day) and on 3rd, 7th and 30th day post treatment. Statistical analysis of data revealed efficacy of herbal gel AV/KPC/10 (M/S Ayurvet Limited) in control and treatment of Ketosis in cattle.

In domestication and development of captive broodstock of tiger shrimp Penaeus monodon mating failures due to poor performance of male brood stock. To overcome this drawback the effect of the testosterone hormone on the performance of male broodstock was evaluated by injecting the hormone at the rate of 5 μg/g. body weight at 5 days interval in test group against control group for a period of 120 days. In both groups male and female were stocked at 1:1 ratio, each animal was tagged and marked, and were fed with clam and squid meat. Mating success, spermataphore structure and duration of moult cycle were observed and compared between two groups. In test group; mating success/animal in females was significantly (P < 0.05) enhanced (65±17.46% versus 47.4±19.49%); spermatophores were solid and intact than those of soft with fluid material in control group; mean moulting period/ animal of males significantly (P < 0.01) reduced (20.85±2.37days versus 26.2±4.25 days). Histological study revealed that the effect of the hormone on Androgenic gland and Y-organ was significant with the hypertrophy of cell and accumulation of secretory globules, respectively. Thus the present study suggests that mating success can be enhanced in tiger shrimp domestication programme through inducing males by hormone for achieving selective breeding between desired/ targeted families/groups.

Mastitis, which has multiple and complex etiology, is a common syndrome among dairy industry. It is widespread in dairy herds and associated with a significant reduction in milk yield resulting in increased costs of production and deteriorated quality of milk and milk products. The initiative of this critical review manuscript was to assess the role of fungi in mastitis of dairy animals. According to some literature data, fungal infections in dairy animals account for 2%–13% of all cases of mastitis in dairy animals. However, the occurrence of mastitis depends on the interaction of host, agent, and environmental factors. The most frequently encountered fungi speices in the pathology of mycotic mastitis in cows are: Candida spp., Aspergillus spp., Trichosporon spp., Cryptococcus spp., Penicillium spp., Cryptococcus neoformans, Rhodotorula spp., and Geotrichum candidum. Mycotic mastitis has become an increasing problem in animals and humans due to the wide use of antibiotics in mastitis therapy. Researches aimed on investigation on mycotic mastitis are required in relation to strict biosecurity practices as well as pattern of antibiotic therapy adopted for the treatment of these cases.

32 multiparous HF crossbred cows in their late gestation (10-15 days prior to parturition) were randomly divided into four groups. Group T0 (n=8) healthy multiparous HF crossbred cows fed standard basal ration & not supplemented with any synthetic or herbal methionine. Group T1 (n=8) cows fed standard basal ration and supplemented with brand A (20g/ day/cattle), Group T2 (n=8) cows fed standard basal ration and supplemented with AV/BMP/35 (herbal methionine supplement) (10g/ day/cattle) and Group T3 (n=8) cows fed standard basal ration and supplemented with AV/BMP/35 (20g/ day/cattle) from 0-15 weeks post partum. Milk yield per group for duration of 0-18 weeks post partum, milk fat %, Milk SNF % and Milk Somatic Cell Count Parameters were recorded during the study at 35th, 70th & 105th day. Results revealed that in AV/BMP/35 supplemented cattles had improved milk production, maintained Fat % and SNF. Results suggest that the AV/BMP/35@ 20g/day/Cattle (M/S Ayurvet Limited, India) was ideal to improve the performance of the cattle.

Taxonomical importance of guard hair has been reported for many animals, but the same has not been studied in detail for several species and sub species of the genus Panthera. Further, although light microscopical studies in this regard are available, there is a lacuna in our knowledge in micro structural features revealed through electron microscopy. The aim of the present study was to have a detailed knowledge on guard hair micro structures with reference to taxonomical applications in two species of the genus Panthera. Distinctness in surface micro structures and internal organisation of cuticle, cortex and medulla of guard hair in Panthera tigris and Panthera pardus as revealed in the current study through scanning electron microscopy suggests the taxonomical significance of guard hair micro structure in these animals.

A cross sectional study was conducted from July to November, 2013 to determine the prevalence of coccidiosis, to identify species of Eimeria and to assess potential risk factors in and around Ambo town, Ethiopia. The study involved ante- and post-mortem examination, mucosal scraping examination using floatation technique, gross and histopathological examination and identification of Eimeria species. Out of 390 examined chickens, 18.7% (73/390) harbored different Eimeria species. Five Eimeria species, Eimeria tenella, Eimeria necatrix, Eimeria brunetti, Eimeria maxima and Eimeria acervulina with the prevalence rate of 62.2%, 18.29%, 10.98%, 6.09% and 2.43%, respectively were identified 82 times from positive chickens. Eimeria tenella was the predominant species while Eimeria maxima and Eimeria acervulina were the least prevalent species. There was no statistical significant difference between age (χ2=0.921, p>0.05), breed (χ2=0.16, p>0.05), sex (χ2=3.609, p>0.05) and management system (χ2=2.245, p>0.05) groups. In conclusion, the present study showed that coccidiosis is an important disease of poultry in the study area. Thus, awareness creation, implementation of appropriate management system, economic effects and further studies on molecular characterization and drug sensitivity of the Eimeria species in the study area were recommended.

In recent years, many studies have been conducted on SERPINA 14, which is one of the molecules suggested to have a role in the occurrence and continuation of pregnancy. In this study, it was aimed to detect the presence of SERPINA 14 mRNA by the reverse transcription-polymerase chain reaction (RT-PCR) in the endometrium of cows in which embryonic mortality and aborted had occurred. In this way, a novel approach can be developed towards embryonic mortality and abortion cases which lead to significant losses in cattle farming. For this purpose, endometrial biopsy samples were obtained from a total of 30 cows which were embryonic mortality (n=10), aborted (n=10) and normal pregnancy (n=10). The presence of SERPINA 14 mRNA in the samples was determined with RT-PCR. Consequently, SERPINA 14 mRNA was detected in the endometrial biopsy samples of all groups. Therefore, it was concluded that the role of SERPINA 14 in continuation of pregnancy could be related to its amount rather than its presence in cows and thereby the present study should be supported by further studies. 

Clinical Indicators of Thiamine deficiency Induced Polioencephalomalacia (PEM) associated with coccidiosis in goat 



Victor I., Wachida N., Terzungwe T., Dzongor J.N., Nsadzetsen G.A., Ogwala E.S., 2014. Clinical Indicators of Thiamine deficiency Induced Polioencephalomalacia (PEM) associated with coccidiosis in goat. The Journal of Veterinary Science. Photon 115, 407-410 


Polioencephalomalacia (PEM), also known as cerebrocortical necrosis, is an important neurologic disease that affects ruminants. The correlation between between coccidia infection, feeding of ruminants with brewed products was examined and suspectedly associated with thiamine deficiency induced PEM. Thiamine deficiency have been well recognized as major etiological factors in PEM. The mechanism of thiamine deficiency associated PEM has been well elucidated. The role of coccidiosis in PEM pathogenesis is linked to the protozoan excessive utilization of thiamine in reproduction and the feeding of ruminants with high molases suppresses the activity of rumen microbes and hence the synthesis of thiamine. The development of clinical PEM is believed to be dependent of thiamine deficiency. This suggests a possible metabolic relationship involvement. Systemic failure to synthesize metabolically requisite levels of Thiamine in the rumen has been suspected in this report. There is increasing evidence of the importance of Thiamine deficiency, host parasite relationship and feeding regime in goats and pathogenesis of Thiamine deficiency induced PEM. This article examined the clinical inter-relationships between the factors mentioned above and PEM, and discuss the potential role of each in the pathogenesis PEM in goats.

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