Molecular Taxonomy Research Award-2015 in Threatened Taxa

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Harshata Pal, Shekhar Ghosh and Swati Sen Mandi are conferred with Molecular Taxonomy Research Award-2015 in Threatened Taxa 


Molecular Authentication of Endangered Medicinal Plant Panax assamicus and Panax bipinnatifidus from North-East India by Species Specific AFLP Marker 

Harshata Pal*, Shekhar Ghosh, Swati Sen Mandi 

Division of Plant Biology, Bose Institute, 93/1, A.P.C. road, Kolkata-700009, India 

Keywords: Panax, AFLP, molecular characterization, species specific marker 

Photon Ignitor: ISJN57438474D772923042015 

Citation: Pal H., Ghosh S., Mandi S.S., 2015. Molecular Authentication of Endangered Medicinal Plant Panax assamicus and Panax bipinnatifidus from North-East India by Species Specific AFLP Marker. The Journal for Threatened Taxa. Photon 113, 138-147. 

Abstract 

Panax assamicus R. N. Banerjee and Panax bipinnatifidus Seem are two most important endangered species rarely found in North-East India. Due to overexploitation this medicinally valuable plant, it is under the threat of extinction in North-East India. Therefore, molecular documentation of few accessions of Panax assamicus and Panax bipinnatifidus from different locations in Meghalay and Arunachal Pradesh was carried out to develop species specific Amplified Fragment Length Polymorphism (AFLP) marker in automated DNA sequencer. Unique set of common alleles generated from electropherogram in AFLP analysis, depending on primer pairs viz. EcoRIACA*/ MSeI-CTA, EcoRIAAG*/MSeICTC, EcoRIACG*/ MSeI-CTG and EcoRI-AGC*/MSeI-CAA, for different accessions of same species are regarded as species specific molecular markers. Both the species Panax assamicus and Panax bipinnatifidus showed 85.25% and 94.55% polymorphism, respectively. The similarity coefficient matrix reflected genetic relatedness among Panax landraces of two species. Cluster I is comprised of four land races of Panax bipinnatifidus and Cluster II is consisted four land races of Panax assamicus in the dendogram, depicting the significance of molecular taxonomy over classical taxonomy to resolute taxonomic complexity of Panax species in NE India. Along with the information on molecular authentication to resist adulteration of these two endangered species the recent research also provides species specific DNA marker relating to catalogue these plant genetic resources. 

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