The Journal for Immunology

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The Journal for Immunology, ISJN: 5743-8480, Impact Index: 5.13, Imprint: Photon, Category: Peer Reviewed Indexed International Journal


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Serum IgE Levels Among Subjects with Symptoms of Airway Disease Mediated By Fungal Allergens 


Evbuomwan L.a* and Okojie R.O.b 


Department of Microbiology, Faculty of Life Sciences, P.M.B. 1154, University of Benin, Benin City, Nigeria 



Abstract 

Aero-allergens are able to elicit an inappropriate and often tissue damaging immunologic reaction mediated by immunoglobulin E (IgE) and termed allergic reaction in the airway. This research work aimed at investigating serum IgE levels in subjects with symptoms of airway diseases. Three hundred (300) subjects with airway disease symptoms were enrolled in this research while seventy subjects without clinical symptoms served as control. One hundred and thirty eight (46%) of the subjects were males while the females were 162 (54%). Blood samples collected from consented subjects, centrifuged at 1000 rpm for 10 min to get serum, was stored at -20 oC until needed. The sera were assayed for IgE level using IgE test kit according to manufacturer’s instruction. Patients sera were reacted with solutions in the test kit and absorbance value were measured using microplate reader. Absorbance value was obtained for standard solution of known IgEconcentration in the test kit and a standard curve of absorbance against IgE concentration was constructed for the standard. The concentration of IgE in the sera of patients was obtained by extrapolation from the standard curve. Patients information was also collected using constructed questionnaire. Mean total serum IgE concentration was found to be 266.05±13.76 and 271.96±13.44 IU/ml in male and female subjects respectively while in the control group, value was 138.99IU/ml. Values ranged from 240.21±45.04 IU/ml among age group 15-25years to 351.25±17.35 IU/ml among group 36-45 years. Prevalence of high serum IgE was found to be 74.7% with serum IgE level above 150 IU/ml. Most airway diseases originate from exposure to inhalational allergens including fungal spores. Therefore, avoiding risk factors associated with increased exposure rate to any potential aero-allergens,is of paramount importance in the prevention of allergic airway diseases. 



Citation: 

Evbuomwan L. and Okojie R.O., 2017. Serum IgE Levels Among Subjects with Symptoms of Airway Disease Mediated By Fungal Allergens. The Journal for Immunology. Photon 108, 139-147  

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Photon Ignitor: ISJN57438480D865417112017










Photon Ignitor: ISJN57438480D793806082015


Citation: Singh C., 2015. Proteomics: A Diagnostic Approach to Autoimmune Diseases. The Journal for Immunology. Photon 107, 135-138


Abstract: Autoimmune diseases affect 3% of the world population, although the diagnosis and classification of autoimmune diseases remain based on clinical examination along with traditional laboratory tests and imaging studies. The main goal of development of genomic and proteomic technologies is that it provides an ability to identify novel biosignatures to diagnose, classify, and guide therapeutic decision making in patients with autoimmune disease. Proteomics technologies enable profiling of autoantibody responses using biological fluids derived from patients with autoimmune disease. They provide a powerful tool to characterize autoreactive B-cell responses in diseases including rheumatoid arthritis, multiple sclerosis, autoimmune diabetes, and systemic lupus erythematosus. In the coming decades, proteomics technologies will broaden our understanding of the underlying mechanisms of and also our ability to diagnose and treat autoimmune disease. This review focuses on the recent advances in proteomics technologies and their application to autoimmune disease so that accurate diagnosis can be achieved so that proper treatment plan can be done.










Fenvalerate Induced Immunotoxicity in Broiler Chicks 


R.V. Ramana Murty, K. Sujatha*, Ch. Srilatha, D. Sreenivasulu, H. Srinivas Naik 


S. V. Veterinary University, Tirupati, India 


R.V. Ramana Murty, K. Sujatha, Ch. Srilatha, D. Sreenivasulu and H. Srinivas Naik are conferred with Max Planck Research Award-2015 in Immunology 


Keywords: Fenvalerate toxicity, Immunosupression, DNFB dermal sensitivity test, Histopathology, Broiler chicks 


Abbreviations: DNFB: Dinitrofluorobenzene 


Photon Ignitor: ISJN57438480D735828042015 


Citation: Murty R.V.R., Sujatha K., Srilatha Ch., Sreenivasulu D., Naik H.S., 2015. Fenvalerate Induced Immunotoxicity in Broiler Chicks. The Journal for Immunology. 107, 130-134. 


Abstract 

The present study was undertaken to evaluate the immunosuppressive effect experimentally induced Fenvalerate toxicity in broiler chicks and its amelioration with Vitamin E. For this study, one hundred fifty apparently healthy unsexed broiler chicks (White Leghorn) were randomly divided into five groups. The control chicks were fed with normal feed without fenvalerate. Group I and II were fed with 20 ppm/kg feed and 40 ppm/kg feed Fenvalerate respectively. Whereas Group III and IV chicks were feed with 20 ppm/kg feed and 40 ppm/ kg feed Fenvalerate respectively along with Vitamin E @ 2.5 ml each (62.5 mgs) in water. Significant decrease in DNFB dermal sensitivity test score in group I, II, III and IV was observed when compared to control. Grossly mild to moderate atrophy of thymus, spleen was noticed. Histopathologically mild to moderate depletion of lymphocytes in germinal centers of spleen and cortex of thymus of all experimental groups was observed when compared to control. Bursa of all experimental groups revealed mild to moderate lymphoid depletion, mild interfollicular fibrous tissue proliferation. The changes in bursa, spleen and thymusalong with decrease in DNFB dermal sensitivity test score are indicating the immunosuppressive effect of the fenvalerate.





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