Journal of Enzymology

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Purification and characterization of phenylalanine ammonia-lyase (PAL) from Leucobryum bowringii Mitt. 


Manoj G Sa, Sumayya S Sa, Bosco Lawarenceb, Murugan Kb


a Department of Botany, VTM NSS College, Dhanuvachapuram, India 

b Plant Biochemistry and Molecular Biology Laboratory, Dept. of Botany, University College, Thiruvananthapuram, Kerala, 695 034, India 


Keywords: 

Phenylalanine ammonia lyase, Characterization, Kinetics, Leucobryum bowringii, Purification


Abbreviations: 

PAL- Phenylalanine ammonia-lyase, pCMB- p-chloro-mercuribenzoate, Hg2+- Mercury, NaBH - sodium borohydride KCN- Potassium cyanide, KAV - partition coefficient


All Rights Reserved with Photon. Photon Ignitor: ISJN60464298D837310082016 


Citation: 

Manoj G.S., Sumayya S.S., Lawarence B., Murugan K.*, 2016. Purification and characterization of phenylalanine ammonia-lyase (PAL) from Leucobryum bowringii Mitt. Journal of Enzymology. Photon 114, 138-149 


Abstract 

Dicranopteris linearis (Burm.F.) Underw. is a desiccation-tolerant forked fern that can tolerate drought. To unravel this feature, the present study was undertaken to assess the role of phenolic com­pounds, its fractionation in the frond in successive periods of desiccation and its localization in the frond cells after enforced desiccation using PEG treatment. Phenolic dis­tribution at the subcellular level was studied using gold particle-conjucated laccase. Cells from different tissues were observed under transmission electron microscope (TEM). The content of phenolic compounds showed positive correlation with desiccation and subsequent rehydration when compared with the control. Remarkable level of phenolic compound deposits was seen in the tissues corroborating with the quantitative data. Fractionation of phenols by RP-HPLC reveals the waxing and waning pattern of phenolic acid such as ferulic acid, hydroxy benzoic acid phloroglucinol and others in the tissue during the different regimes of desiccation. Phenolics were noticed in the vacuoles of all cells and also along the cell walls. The results suggest the caliber of the fern to withstand desiccation tolerance by accumulating phenolics. The in situ location of phenolics further reveals the protective chemical defense of the compound against environmental constraints. Further studies are warranted to analyze the antioxidant enzymes and compounds in the fern in relation to desiccation.




Enzymes Role in Our Life 


Mahendra R. Pakhale*, Varshay R. Pakhale, Dr. Harshal Pachapor, Dr. S. Pramanik 


Department of Biochemistry, S.V.N. Govt. Medical College, Yavatmal (MS), India 


Mahendra R. Pakhale, Varshay R. Pakhale, Dr. Harshal Pachapor and Dr. S. Pramanik are conferred with Richard Kuhn Research Award-2015 in Enzymology


Keywords: 

Enzyme, drugs, inhibitors, art, history, industry 


Photon Ignitor: ISJN60464298D810430122015 


Citation: 

Pakhale M.R., Pakhale V.R., Pachapor H., Pramanik S. 2015. Enzymes Role in Our Life. Journal of Enzymology. Photon 113, 130-137 


Abstract 

Life as we know it heavily relies on biological catalysis, in fact, in a very nonromantic version of it, life could be considered as a series of chemical reactions, regulated by the guarding principles of thermodynamics. The history of enzy-mology is as old as humanity itself, and dates back to the ancient times. This paper is dedicated to these early moments of this remarkable science that touched our lives in the past and will make life a lot more efficient for humanity in the future. There was almost always a delicate, fundamen-tally essential relationship between mankind and the enzymes. Challenged by a very alien and hostile Nature full of predators, prehistoric men soon discovered the medicinal properties of the plants, through trial and error. In fact, they accidently discovered the enzyme inhibitors and thus, in crude terms, kindled a sparkling area of research. These plant-derivatives that acted as enzyme inhibitors helped pre-historic men in their pursuit of survival and protection from predators; in hunting and fishing Later in history, while the underlying purposes of survival and increasing the quality of life stayed intact, the ways and means of enzymology experi-enced a massive transformation, as the ‘trial and error’ methodology of the ancients is now replaced with rational scientific theories.