PUBLICATIONS 發表

2025

Hu, Y.-Y.#, Chang, Y.-C.#, Tsai, Y.-Y.#, Chang, H.-Y., Chi, P.* & Li, H.-W.*,  SWI5-SFR1 Reduces RAD51 Recombinase Extending Units during Filament Assembly, Nucleic Acids Res., 53, gkaf676 (2025). [LINK]

台大理學院報導：https://www.science.ntu.edu.tw/news-977/

Wang, M. F., Li, M.-Y., Yang, Y.-C., Chuang, Y.-C., Tsai, C.-Y, Nguyen, B. M.-C., Ma, L., Lin, S.-W., Li, H.-W., Smith, G. R. & Chi, P.*, Mug20-Rec25-Rec27 binds DNA and enhances meiotic DNA break formation via phase-separated condensates, Nucleic Acids Res., 53, gkaf123 (2025). [LINK]

2024

Wei, C.-D.#, Chang, H.-Y.#, Lu, C.-H., Chang, C.-C., Furukohri, A., Mwaniki, S., Shinohara, A., Chi, P.* & Li, H.-W.*, Mei5-Sae3 stabilizes Dmc1 nucleating clusters for efficient Dmc1 assembly on RPA-coated single-stranded DNA, Nucleic Acids Res., 52, 11768 (2024). [LINK]

台大理學院報導：https://www.science.ntu.edu.tw/news-948/

Asia Research News 報導：https://admin.asiaresearchnews.com/content/international-collaboration-team-reveals-key-mechanism-regulating-dna-recombination

Peng, J.-C., Chang, H.-Y., Sun, Y. L., Prentiss M., Li, H.-W. & Chi, P.*, Hop2-Mnd1 functions as a DNA sequence fidelity switch in Dmc1-mediated DNA recombination, Nature Comm., 15, 9266 (2024). [LINK]

台大報導: 基因重組新發現 臺大與哈佛破解遺傳多樣性之謎 研究成果發表於Nature Communications

2023

Lee, W., Iwasaki, H., Tsubouchi, H.* & Li, H.-W.*, Hop2-Mnd1 and Swi5-Sfr1 stimulate Dmc1 filament assembly using distinct mechanisms, Nucleic Acids Res., 51, 8550 (2023). [LINK]
台大理學院報導：https://www.science.ntu.edu.tw/news-888/
東京工業大學報導：https://www.titech.ac.jp/news/2023/067171 (Japanese version) https://www.titech.ac.jp/english/news/2023/067190 (English version)

Guh, C.-L., Lei, K.-H., Chen, Y.-A., Jiang, Y.-Z., Chang, H.-Y., Liaw, H., Li, H.-W., Yen, H.-Y. & Chi, P.*, RAD51 paralogs synergize with RAD51 to protect reversed forks from cellular nucleases, Nucleic Acids Res., 51, 11717-11731 (2023). [LINK]

2021

Lei, K.-H.#, Yang, H.-L.#, Chang, H.-Y., Yeh, H.-Y., Nguyen, D. D., Lee, T.-Y., Lyu, X., Chastain, M., Chai, W., Li, H.-W.* & Chi, P.*, “Crosstalk between CST and RPA regulates RAD51 activity during replication stress” Nature Comm., 12, 6412 (2021). [LINK]

台大報導: 臺大團隊解開DNA複製修復機制研究成果發表於Nature Communications

Lee, T.-Y., Li, Y.-C., Lin, M.-G., Hsiao, C.-D.* & Li, H.-W.*, "Single-Molecule Binding Characterization of Primosomal Protein PriA Involved in Replication Restart", Phys. Chem. Chem. Phys., 23, 13745-13751 (2021). [LINK]

Lu, C.-H., Lan, W.-H. & Li, H.-W.*, "Single-Molecule Tethered Particle Motion Studies on the DNA Recombinase Filament Assembly and Disassembly", Methods Mol. Biol., 2281, 135-149 (2021). [LINK]

Lin, Y.-Y., Li, M.-H., Chang, Y.-C., Fu, P.-Y., Ohniwa, R. L., Li, H.-W.* & Lin, J.-J.*, “Dynamic DNA Shortening by Telomere-Binding Protein Cdc13” J. Am. Chem. Soc., 143, 5815-5825 (2021).  [LINK]

Li, W.-C.#, Lee, C.-Y.#, Lan, W.-H.#, Woo, T.-T., Chen, C.-Y., Yeh, H.-Y., Chang, H.-Y., Liu, H.-C., Chen, C.-L., Chuang, C.-N., Hsueh, Y.-P., Li, H.-W.*, Chi, P.*, Wang, T.-F.*, “Trichoderma reesei Rad51 tolerates mismatches in hybrid meiosis with diverse genome sequences” Proc. Natl. Acad. Sci. U.S.A., 118, e2007192118 (2021). [LINK]

Luo, S.-C., Yeh, H.-Y., Lan, W.-H., Wu, Y.-M., Yang, C.-H., Chang, H.-Y., Su, G.-C., Lee, C.-Y., Wu, W.-J., Li, H.-W., Ho, M.-C.*, Chi, P.*, Tsai, M.-D.* “Identification of fidelity-governing factors in human recombinases DMC1 and RAD51 from cryo-EM structures” Nature Comm., 12, 115 (2021). [LINK]

台大報導: 遺傳多樣性的奧妙 為什麼基因不會百分百遺傳？冷凍電顯看穿DNA不完美配對的結構

2020

Chang, H.-Y., Lee, C.-Y., Lu, C.-H., Lee, W., Hang, H.-L., Yeh, H.-Y., Li, H.-W.* & Chi, P.* "Microcephaly family protein MCPH1 stabilizes RAD51 filaments" Nucleic Acids Res., 48, 9135-9146 (2020). [LINK]

台大報導: http://host.cc.ntu.edu.tw/sec/schinfo/epaper/article.asp?num=1445&sn=17576

"單分子光鉗系統之建置校正及生醫應用", 張晏展，周冠廷，吳順德，李弘文，科儀新知，223, 77-90 (2020).[LINK]

Lan, W.-H.#, Lin, S.-Y.#, Kao, C.-Y., Chang, W.-H.,Yeh, H.-Y., Chang, H.-Y., Chi, P.* & Li, H.-W.* "Rad51 Facilitates Filament Assembly of Meiosis-Specific Dmc1 Recombinase" Proc. Natl. Acad. Sci. U.S.A., 117, 11257-11264 (2020). [LINK]

台大報導: http://host.cc.ntu.edu.tw/sec/schinfo/epaper/article.asp?num=1436&sn=17469

NTU highlights: http://ntuhighlights.ntu.edu.tw/pdf/078.pdf

Tirtom, N.-S., Hsu, Y. & Li, H.-W.* "Polyamines Stimulate RecA-mediated Recombination by Condensing Duplex DNA and Stabilizing Intermediates" Phys. Chem. Chem. Phys., 22, 11928-11935 (2020). [LINK]

2019 and before

Lin, Y.-H., Chu, C.-C., Fan, H.-F., Wang, P.-Y., Cox, M. M.* & Li, H.-W.* "A 5'-to-3' Strand Exchange Polarity is Intrinsic to RecA Nucleoprotein Filaments in the Absence of ATP Hydrolysis" Nucleic Acids Res., 47, 5126-5140 (2019). [LINK]

Lu, C.-H., Yeh, H.-Y., Su, G.-C., Ito, K., Kurokawa, Y., Iwasaki, H.*, Chi, P.* & Li, H.-W.* "Swi5-Sfr1 Stimulates Rad51 Recombinase Filament Assembly by Modulating Rad51 Dissociation" Proc. Natl. Acad. Sci. U.S.A., 115 (43) E10059-E10068 (2018).[LINK]

台大報導:http://www.ntu.edu.tw/spotlight/2018/1552_20181019.html

日本東京工業大學報導: https://www.titech.ac.jp/english/news/2018/042590.html

Cho, C.-C., Chung, C. & Li, H.-W.* "How Chi Sequence Modifies RecBCD Single-Stranded DNA Translocase Activities." ChemPhysChem.,19, 243 (2018).[LINK]

Lu, C.-H#, Chang, T.-T#, Cho, C.-C., Lin, H.-C. & Li, H.-W.* "Stable Nuclei of Nucleoproteiin Filament and High ssDNA Binding Affinity Contribute to Enhanced RecA E38K Recombinase Activity", Sci. Rep. 7, 14964 (2017). (#Equal contribution) [LINK]

Wu, H.-Y#, Lu, C.-H.# & Li, H.-W.* “RecA-SSB Interaction Modulates RecA Nucleoprotein Filament Formation on SSB-Wrapped DNA”, Sci. Rep., 7, 11876 (2017). (#Equal contribution) [LINK]

Kao, W.-S. & Li, H.-W.*, “An Efficient Bead-Captured Denaturation Method for Preparing Long Single-stranded DNA”, J. Chin. Chem. Soc., 64, 1065  (2017). [LINK]

Lu, C.-H & Li, H.-W.* “DNA with Different Local Torsional States Affects RecA-Mediated Recombination Progression”, ChemPhysChem., 18, 584 (2017).[LINK]

Chen, Y.-F., Lu, C.-Y., Lin, Y.-C., Yu, T.-Y., Chang, C.-P., Li, J.-R., Li, H.-W. & Lin, J.-J.* “Modulation of yeast telomerase activity by Cdc13 and Est1 in vitro”, Sci. Rep., 6, 34104 (2016).[LINK]

Li, J.-R., Lu, C.-Y., Lin, J.-J.* & Li, H.-W.* “Multiple Pif1 helicases are required to sequentially disrupt G-quadruplex structure and unwind duplex DNA”, Biochem Biophys Res Commun, 473, 1235-9 (2016).[LINK]

Carter, A.R., Seaberg, M.H., Fan, H.-F., Sun, G., Wilds, C.J., Li, H.-W. & Perkins, T.T. “Sequence-dependent nanometer-scale conformational dynamics of individual RecBCD-DNA complexes”, Nucleic Acids Res., 44, 5849-60 (2016).[LINK]

Wang, C.-C., Wu, S.-M., Li, H.-W.* & Chang, H.-T.* “Biomedical Applications of DNA-Conjugated Gold Nanoparticles”, ChemBioChem, 17, 1052-62 (2016).[LINK]

Piechura, J. R., Tseng, T.-L., Hsu, H.-F., Byrne, R.T., Battista, J. R., Li, H.-W. & Cox, M. M.* “Directed Evolution of Extreme Resistance to Ionizing Radiation: How RecA Protein Adaptation Contributes”, DNA Repair, 26, 30-43 (2015).[LINK]

Li, J.-R., Yu, T.-Y., Chian, I.-C., Lu, C.-Y., Lin, J.-J.* & Li, H.-W.*, “Pif1 Regulates Telomere Length by Preferentially Removing Telomerase from Long Telomere Ends” Nucleic Acids Res., 42, 8527-8536 (2014). [LINK]

Yuan, Z, Chen, Y.-C., Li, H.-W.* & Chang, H.-T.*, “Fluorescent silver nanoclusters stabilized by DNA scaffolds” Chem. Comm., 50, 9800-9815 (2014). [LINK]

Wu, H.-Y. & Li, H.-W.*, “Crowding Alters Dynamics and Length of RecA Nucleoprotein Filaments in RecA-Mediated Strand Exchange” ChemPhysChem, 15, 80-84 (2014). [LINK]

Su, G.-C., Chung, C.-I., Liao, C.-Y., Lin, S.-W., Tsai, C.-T., Huang, T., Li, H.-W. & Chi, P.*, “Enhancement of ADP Release From the RAD51 Presynaptic Filament by the SWI5-SFR1 Complex” Nucleic Acids Res.,42, 349-358 (2014). [LINK]

Chung, C. & Li, H.-W.*, “Direct Observation of RecBCD Helicase as Single-Stranded DNA Translocases” J. Am. Chem. Soc. 135, 8920-8925 (2013). [LINK]

Lu, Y.-W., Huang, T., Tsai, C.-T., Chang, Y.-Y., Li, H.-W., Hsu, C.-H. & Fan, H.-F.*, “Using Single-Molecule Approaches to Study the Archaeal DNA Binding Protein Alba1” Biochemistry, 52, 7714-7722 (2013). [LINK]

Liao, S.-M., Wu, H.-Y. & Li, H.-W.*, “Salt-Dependence of Homology Searching Step by RecA Nucleoprotein Filaments”, J. Chin. Chem. Soc. 60, 695-698 (2013). [LINK]

Liu, S.-W., Chu, J.-F., Tsai, C.-T., Fang, H.-C., Chang, T.-C., Li, H.-W.*, “Assaying the binding strength of G-quadruplex ligands using single-molecule TPM experiments” Anal. Biochem. 436, 101-108 (2013). [LINK]

Ho, L.-C., Ou, C.-M., Li, C.-L., Chen, S.-Y., Li, H.-W.* & Chang, H.-T.*, “Sensitive pH probes of retro-self-quenching fluorescent nanoparticles”, J. Mater. Chem. B 1, 2425-2432 (2013). [LINK]

Lo, Y.-H., Liu, S.-W., Sun, Y.-J., Li, H.-W.* & Hsiao, C.-D.*, "Mutations Altering the Interplay between GkDnaC Helicase and DNA Reveal an Insight into Helicase Unwinding", PLoS ONE, 6, e29016 (2011). [LINK]

Hsu, H.-F., Ngo, K. N., Chitteni-Pattu, S., Cox, M. M. & Li, H.-W.*, "Investigating Deinococcus radiodurans RecA protein filament formation by real-time single-molecule approaches", Biochemistry, 50, 8270−8280 (2011). [LINK]

Fan, H.-F., Cox, M. M. & Li, H.-W.*, "Developing Single-Molecule TPM Experiments for Direct Observation of Successful RecA-Mediated Strand Exchange Reaction", PLoS ONE 6, 7 (2011). [LINK]

Chu, J.-F., Chang, T.-C., and Li, H.-W.*, "Single-Molecule TPM Studies on the Conversion of Human Telomeric DNA", Biophysical J. 98, 1608 (2010). [LINK]

Perkins, T. T., and Li, H.-W,, "Single-Molecule Studies of RecBCD", in Helicases, Methods in Molecular Biology 587, 155 (2010), edited by Abdelhaleem, Humana Press (Book chapter).

單分子拴球實驗在生醫研究的應用， 陳晏誠、 范秀芳、 李弘文， 科儀新知, 171,38-43 (2009).

H.-F. Fan & Li, H. -W.*, “Studying RecBCD Helicase Translocation along Chi-DNA Using Tethered Particle Motion with a Stretching Force”, Biophysical J, 96, 1875 (2009). [LINK]

Perkins, T. T., Li, H. -W., Dalal, R. V., Gelles, J. & Block, S. M. “Forward and Reverse Motion of Single RecBCD Molecules along DNA”, Biophysical J, 86, 1640 (2004). [LINK]