Research Interest
Molecular biophysics, single-molecule biochemistry, protein - DNA interactions, nano-bioscience
Our major interest is to understand important biological processes at the single-molecule level. Elucidating the functional mechanisms of these processes is essential in understanding the molecular basis of the associated disease processes. Single molecule techniques observe the distributions and time trajectories of individual molecules, information that is lost in the ensemble average, and thus provide valuable insights into the mechanisms of enzyme function.
We are especially interested in biological molecular machines involved in DNA recombination and repair. The goal is to investigate the mechanistic details of these DNA-processing proteins, to explore the regulatory architecture, and to elucidate the general mechanism of protein/DNA interactions. This research lies at the interface of biology, chemistry and physics, and the tools we use include various single molecule light microscopy and fluorescence techniques, and biochemistry methods. This nano-bioscience approach also involves in developing new physical techniques to detect bio-macromolecular motion with nanometer precision.
This laboratory is funded by
National Science and Technology Council
and National Taiwan University
研究興趣
分子生物物理、單分子生物化學、蛋白質與DNA間的交互作用、奈米生物學
本實驗室主要利用單分子光學顯微鏡的方法，來研究重要的生化過程與其機制。闡明生化過程的工作機制，可助於了解與生化分子相關疾病中，分子間作用的形態。單分子實驗技術可觀察單一分子的作用方式及其隨時間的變化軌跡。更重要的是，在平常大量分子的實驗條件下，得到的數據與效應是整體分子的平均值；而單分子實驗技術提供在一般巨觀實驗被平均掉、看不到的資訊，因此可讓我們對於酵素作用的機制有更有效的研究。
本實驗室研究負責DNA重組與修復的酵素組。我們的目標在於研究這些處理DNA的蛋白質之作用細節、探索其中用以調控的結構、以及說明蛋白質與DNA交互作用的主要機制。單分子生物物理化學的研究跨及生物學、化學與物理學等領域。我們所使用的工具有各式可觀測奈米尺度運動的單分子顯微技術、螢光顯微技術與各種生物化學方法。我們也利用奈米生物學的概念，發展在奈米解析度下，偵測生物大分子運動的新穎物理、化學技術。
本實驗室經費由
國科會及國立台灣大學贊助