The PacBio RS system can be used for de novo assembly, targeted sequencing, and base modification detection. There are two main sequencing options: Continuous Long Reads (CLRs) and Circular Consensus (CCS). Multiple size options can be used with these sequencing options.
We can prepare libraries of sizes; 500bp, 1kb, 2kb, 5kb, 10kb as well as amplicons without shearing. Longer inserts (10kb) are appropriate for CLR and short inserts (up to 3kb) are appropriate for CCS. Additionally CLR is appropriate for de novo assembly, while CCS is appropriate for targeted sequencing.
There are also movie sequencing options associated with the read types:
- One 120-minute movie will maximize the read length but only capture half (~30,000 reads) of the ZMWs (reaction wells) on a SMRTcell (where sequencing takes place). This is recommended for CLR with a maximum read length > 20kb (average 4kb).
- Two 55-minute movies will have a greater number of shorter reads (~60,000 reads). This is recommended for CCS with a maximum read length of > 9kb (average 3kb).
- These standard protocols can be modified upon request.
500bp or 1kb
The number of SMRTcells you’ll need to run depends on the application. For more information please discuss your project with a HTSF PacBio tech and the bioinformatican in charge of PacBio data analysis.
If you are interested in pricing, wait time details, or have submission questions regarding PacBio sequencing please contact:
- Piotr Mieczkowski, HTSF Director (email@example.com)
- Corbin Jones, HTSF faculty adviser (firstname.lastname@example.org)
For PacBio Library prep questions please contact:
For bioinformatics questions regarding PacBio sequencing please contact:
- Tristan De Buysscher (email@example.com)
For questions regarding PacBio systems and technology please contact:
- George Yuan (firstname.lastname@example.org)
These are general instructions (all samples)
All samples submitted are assumed to be measured by a flourometer (e.g. Qubit). However, if sample concentrations have been measured using a spectrophotometer (e.g. Nanodrop) please compensate for overestimation and double the input amount.
*Please include information on the size of the material being submitted.