The section below is a feed for news about the HTSF and other items pertinent to genomic research. If you wish to subscribe to this feed an RSS link is provided below.
After a very delicate, time-consuming move from it's temporary operating site in the HTSF's off-campus Sequencing Facility, we're pleased to announce that the PacBio RS is up and running again in the new Genome Sciences Building!
So please feel free to start submitting samples again that you wish to have processed with PacBio technology. More information is available on the HTSF's PacBio RS page.
Please join us on University Day, October 12, 2012 for the Opening Symposium and Celebration of UNC's new state-of-the art Genome Sciences Building.
The GSB has created 210,000 square feet of vital new space for genomics research, housing more than 400 faculty and researchers in the heart of the UNC campus. The open modern architectural design of the building was conceived with interdisciplinary research in mind, and integrates faculty from the departments of Biology, Chemistry, Statistics and Operations Research, and Computer Science, as well as serving as a home for genome-related core facilities. The certified LEED “green” certified building also features two 450-seat and 250-seat auditoriums and an entire floor of rooftop greenhouses to support research in plant genomics.
Everyone is invited to attend the GSB symposium, but registration is required to attend the lunch and to ensure that there is sufficient seating and refreshments.
If you would like to present a poster, you will need to submit a poster abstract. You can submit your abstract when you register or by emailing it to John Cornett, making sure to provide your full name, affiliation and the email address used to register.
- All poster abstracts must be received by September 28, 2012.
- Symposium registration will close on October 5, 2012.
Go to the UNC Events Registration webpage. A dropdown box at the top of the page will narrow the listings by sponsor. Select Carolina Center for Genome Sciences as the sponsor. Then click on the listing for Genome Science Building Opening Symposium and Dedication to go to the registration page for this event. Click the "Register for this event" button at the bottom of that page. If you are affilitated with UNC and have an Onyen, you can login to register using your UNC Onyen. If you do not have a UNC Onyen, you can continue to register without logging in by simply providing your email address.
You will receive a confirmation email upon completing registration. For further information, please contact John Cornett.
Official Announcement [link]
In late August 2012, the High-Throughput Sequencing Facility's library preparation core (currently located in GMB) will move to the new Genome Sciences Building (GSB).
While we expect our library preparation facility to shut down for at least two weeks, sequencing of prepared libraries will continue as usual. Additionally, we have cross-trained several members of our staff in the preparation of libraries so that we are able to quickly process and sequence submitted samples after the move.
During this time, we expect minimal disruption of miSeq or Ion Torrent related services.
If you have questions, please don’t hesitate to contact us.
Corbin Jones and Piotr Mieczkowski
The HTSF's new PacBio RS system is up and running!
For those interested in taking advantage of this cutting-edge sequencing technology for use in their research, we've just posted some general information and submission requirements on the HTFS's PacBio RS webpage. At the bottom of the page you can also find printable instructions and submission forms available for download.
If you have any further questions about the HTSF's PacBio sequencing services, pricing, and turnover times, please contact the appropriate HTSF technical support staff.
We've updated and posted our protocols for submitting samples and test requests on the Forms & Guides page.
From now on, this link can be found in the navigation menu under Services We Offer. A downloadable PDF copy of this protocol is also available below.
Of course, please contact us with any questions you may have.
The HTSF's Ion Torrent Sequencer provides excellent "sequencing by synthesis" capabilities, yielding data quickly and affordably.
The 2.5 day submission-to-data turnover time consists of:
- library preparation (1 day)
- emulsion PCR (1 day)
- sequencing (1/2 day)
Currently, we offer runs with Ion 314 and 316 Chips (runs using Ion 318 Chips will be available soon).
An Ion 314 Chip run yields:
An Ion 316 Chip run yields:
- ~40 Mb or more per run
- ~200,000+ reads at 200 bp length
- ~200 Mb per run
- 1,000,000+ reads at 200 bp length
While the figures above provide an idea of our current output per run, Ion Torrent runs are still being optimized and we expect higher-quality output in the coming weeks.
A price sheet for all types of runs (314/316/318) is available for download below. If you have any other questions about the HTSF's Ion Torrent Sequencer, please contact Nick Schuch.
We want to wish all of our customers and collaborators a most happy and prosperous Lunar New Year.
We hope the Year of the Dragon brings great things for you and your family, and in your research.
Illumina's CEO introduced the HiSeq 2500, which some analysis predict will make sequencing the human genome for under $1,000 a reality. On the same day, Life Tech announced the released the Ion Proton Sequencer. In addition to being able to sequence a genome in less than $1,000, the company claims that the procedure can be performed in less than a day.
Both systems are expected to be available later this year.
Our sequencing and support services will resume on Tuesday, January 3, 2012. We look forward helping you and your research get off to a great start in 2012.
Happy New Year!
Service at the HTSF equipment will be slowing down during the university holiday break (Dec 22 - Jan 2). Support for PacBio, Ion Torrent and Illumina systems will also be unavailable or limited during this time.
We will return to normal service on Jan 3, 2012.
We wish you the Happiest of Holidays!