![]() We combined cryoEM and mass spectrometry to determine the atomic
structure of a feline coronavirus spike protein. We demonstrated the ability to directly
visualize post-translationally modified glycan structures that shield most of the
viral spike protein surface, which has long been a major challenge in
structural biology. In light of the
resurgence of SARS, which is transmitted by coronaviruses, our technological
advancement can be readily applied to examine the molecular details of
infectious viral glycoproteins that are involved in host recognition and viral
entry and to aid vaccine developments. This work has been published in PNAS https://www.pnas.org/content/early/2020/01/02/1908898117 |
![]() Congratulations to Manoj Kumar who demonstrated some of the very unusual characteristics of the 52 knotted human ubiquitin C-terminal hydrolases (UCHs) in withstanding the ATP-fueled mechano-unfolding-coupled proteolysis by E. coli AAA+ protease, ClpXP. This work demonstrates the stabilizing contribution of the 52 knot to the mechanostability (Sriramoju et al., BBA - Proteins and Proteomics).
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