ABO-Rh Blood Typing With Synthetic Blood

Question

Can DNA testing be used to determine if babies were switched at birth?

Background Research

Source 1,Source 2, and Source 3 there is more background research at the bottom of the page on the word document.

Hypothesis

I believe DNA testing can be used to determine if babies were switched at birth.

Materials

SYNTHETIC BLOOD SAMPLES

1, 2, 3 AND 4

1 Synthetic anti-A serum

1 Synthetic anti-B serum

1 Synthetic anti-Rh serum

4 BLOOD TYPING SLIDES

3 PACKS OF DIFFERENT

COLOR MIXING STICKS

4 DROPPERS

(ON THE BOTTOM OF THIS PAGE IS A POWERPOINT WHICH HAS PICTURES OFF ALL THINGS NEEDED TO CONDUCT THIS EXPERIMENT)

Safety Regulations

Set up a workstation where students can obtain the synthetic blood and synthetic anti-serum samples . After the tests of all four blood types have the students clean their blood typing slides and droppers thoroughly.

2. Add a drop of synthetic anti-A (blue) to the well labeled A. Replace the cap.

3. Add a drop of synthetic anti-B serum (yellow) to the well labeled B. Replace the cap.

4. Add a drop of synthetic anti-Rh serum (clear) to the well labeled Rh. Replace the cap.

5. Using a different color mixing stick for each well (blue for anti-A, yellow for anti-B, white for anti-Rh), gently stir the synthetic blood and anti-serum drops for 30 seconds. Remember to discard each mixing stick after a single use to avoid contamination of your samples.

6. Carefully examine the thin films of liquid mixture left behind. If a film remains uniform in appearance, there is no agglutination. If them sample appears granular, agglutination has occurred. Determine the blood type of the sample using the data table below. Answer yes or no as to whether agglutination occurred in each sample. A positive agglutination reaction indicates the blood type.

7. Record the results for the first blood sample in the data table.

8. Thoroughly rinse the blood typing slide, then repeat steps 1 through 7 for synthetic blood samples 2, 3, and 4.