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Introduction:
Introduction:
Hello my name is Stephen, im from Harmony Schools and my project is Strawberry DNA Extraction.DNA is a double stranded macromolecule composed of nucleotide bases pairing Adenine with Thymine and Guanine with Cytosine. DNA can be extracted from cells by a simple technique with household chemicals, enabling you to see strands of DNA with the naked eye.
Hello my name is Stephen, im from Harmony Schools and my project is Strawberry DNA Extraction.DNA is a double stranded macromolecule composed of nucleotide bases pairing Adenine with Thymine and Guanine with Cytosine. DNA can be extracted from cells by a simple technique with household chemicals, enabling you to see strands of DNA with the naked eye.
Purpose:
Purpose:
To extract DNA from the fruit of a strawberry.
To extract DNA from the fruit of a strawberry.
Safety Precautions:
Safety Precautions:
* Wear Apron,Gloves & Safety Goggles.
* Wear Apron,Gloves & Safety Goggles.
Materials / Equipment :
Materials / Equipment :
1. heavy duty zip-lock baggie
1. heavy duty zip-lock baggie
2. strawberries (fresh or frozen and thawed)
2. strawberries (fresh or frozen and thawed)
3. coffee filter
3. coffee filter
4. 100 ml beaker
4. 100 ml beaker
5. glass rod or pipette tip
5. glass rod or pipette tip
6.liquid dishwashing detergent
6.liquid dishwashing detergent
7.alcohol
7.alcohol
8.salt
8.salt
Procedure:
Procedure:
1. Place strawberries in a zip lock baggie & smash the strawberries until they become liquidy.
1. Place strawberries in a zip lock baggie & smash the strawberries until they become liquidy.
2. Add 1 tablespoon of liquid dishwashing detergent ,& sprinkle in some salt.
2. Add 1 tablespoon of liquid dishwashing detergent ,& sprinkle in some salt.
3. Mush again to mix the added ingredients.
3. Mush again to mix the added ingredients.
4. Filter solution through the coffee filter into the beaker.
4. Filter solution through the coffee filter into the beaker.
5. After filtering the chunks of strawberry from the liquidy solution ,add alchohal to the strawberry solution .
5. After filtering the chunks of strawberry from the liquidy solution ,add alchohal to the strawberry solution .
6. At the interface, you will see the DNA precipitate out of solution and float to the top. You may spool the DNA on your glass rod or pipette tip.
6. At the interface, you will see the DNA precipitate out of solution and float to the top. You may spool the DNA on your glass rod or pipette tip.
7. Spool the DNA by dipping a pipette tip or glass rod into the tube right where the extract layer & alcohol are in contact with each other. With your tube at eye level, twirl the rod & watch as DNA strands collect.
7. Spool the DNA by dipping a pipette tip or glass rod into the tube right where the extract layer & alcohol are in contact with each other. With your tube at eye level, twirl the rod & watch as DNA strands collect.
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