Publications

Fu B, Xu T, Cui Z, Ng HL, Wang K, Li J, Li QX. (2017). Accessibility into catalytic site confining substrate selectivity: Mutation of phenylalanine-223 to leucine enhances transformation of benzo[a]pyrene by ring-hydroxylating dioxygenase in Sphingobium sp. FB3. Submitted.

Forest K, Alfulaij N, Arora K, Lawrence JLM, Yoshikawa G, Ng HL, Hruby VJ, Nichols RA. (2017). Neuroprotection against β-Amyloid toxicity by a non-toxic endogenous N-terminal β-amyloid fragment and its active hexapeptide core sequence. Submitted.

Buchko GW, Echols N, Flynn EM, Ng HL, Stephenson S, Kim HB, Myler PJ, Terwilliger TC, Alber T, Kim CY. (2017). Structural and biophysical characterization of Rv0577, a protein associated with neutral red staining of virulent strains of Mycobacterium tuberculosis and homolog of the Streptomyces coelicolor protein KbpA. Biochemistry. DOI: 10.1021/acs.biochem.7b00511 

Peres R, Furuya H, Hokotan K, Shimizu Y, Chan O, Pagano I, Dyrskjot L, Jensen J, Malmstrom P, Segersten U, Ng HL, Janku F, Rosser C. (2017). Angiogenin contributes to bladder tumorigenesis by facilitating p53-mediated activation of MMP2. Submitted.

Bajinting A, Ng HL. (2017). Recombinant expression in E. coli of human FGFR2 with its transmembrane and extracellular domains. PeerJ. 5:e3512.dx.doi.org/10.7717/peerj.3512

Kim J, Ng HL. (2017). Screening and identifying membrane proteins favorable for crystallization. Current Protocols Protein Sci. In press.

Vidad AR, Macaspac S, Ng HL. (2016). Locating the ligand binding sites for the G-protein coupled estrogen receptor (GPER) using combined information from docking and sequence conservation. http://biorxiv.org/content/early/2016/06/29/061051

Ng HL. (2016). Simulations reveal increased fluctuations in estrogen receptor-alpha conformation upon antagonist binding. J. Molec.Graphics Modelling. 69, 72.  http://dx.doi.org/10.1016/j.jmgm.2016.08.009

Ng HL, Lin MZ. (2016). Structure-guided wavelength tuning in far-red fluorescent proteinsCurrent Opin. Struct. Biol. 39, 124. dx.doi.org/10.1016/j.sbi.2016.07.010

Chu J, Oh Y, Sens A, Ataie N, Dana H, Macklin JJ, Laviv T, Welf ES, Dean KM, Zhang F, Kim BB, Tang CT, Hu M, Baird MA, Davidson MW, Kay MA, Fiolka R, Yasuda R, Kim DS, Ng HL, Lin MZ. (2016). A bright cyan-excitable orange fluorescent protein facilitates dual-emission microscopy and enhances bioluminescence imaging in vivo. Nature Biotechnol. doi: 10.1038/nbt.3550. link

Ataie N, Xiang J, Cheng N, Brea EJ, Lu W, Scheinberg DA, Liu C, Ng HL. (2016). Structure of a TCR-mimic antibody with target predicts pharmacogenetics. J. Mol. Biol. doi: 10.1016/j.jmb.2015.12.002. link

Kim J, Kagawa A, Kurasaki K, Ataie N, Cho IK, Li QX, Ng HL. (2015). Large scale identification of membrane proteins with properties favorable for crystallization. Protein Sci. doi: 10.1002/pro.2766. link

Ataie N, Ng HL. (2014). X-ray crystallography: A science of truth and beauty. Science Vision. 20, 49.

Chu JHaynes RCorbel SYLi PGonzález-González EBurg JSAtaie NJLam AJCranfill PJBaird MADavidson MWNg HLGarcia KC ,Contag CHShen KBlau HMLin MZ. (2014). Non-invasive intravital imaging of cellular differentiation with a bright red-excitable fluorescent protein. Nature Methods. doi: 10.1038/nmeth.2888. link

Tosha T, Behera RK, Ng HL, Bhattasali O, Alber T, Theil EC. (2012). Ferritin protein nanocage ion channels: gating by N-terminal extensions. J. Biol. Chem. 287, 13016. link

Lombana TN, Echols N, Good MC, Thomsen ND, Ng HL, Greenstein AE, Falick AM, King DS, Alber T. (2010). Allosteric activation mechanism of the Mycobacterium tuberculosis receptor Ser/Thr protein kinase, PknB. Structure. 18, 1667. link

Tosha T*, Ng HL*, Bhattasali O, Alber T, Theil EC. Moving metal ions through ferritin protein nanocages: the journey from three-fold pores to catalytic sites. (2010).  J. Am. Chem. Soc.  132, 14562. (*Co-lead authors). link

Lang PT*, Ng HL*, Fraser JS, Corn J, Echols N, Sales M, Holton J, Alber T. (2010). Observation of protein conformational polymorphism in crystallographic electron density maps. Protein Sci. 19, 1420. (*Co-lead authors). link

Lin MZ, McKeown MR, Ng HL, Aguilera T, Shaner NC, Adams SR, Ma W, Alber T, Tsien RY. (2009). Autofluorescent proteins with excitation in the optical window for intravital imaging in mammals. Chemistry & Biology 16, 1169. link

Bertin A, McMurray MA, Grob P, Park SS, Garcia G, Patanwala I, Ng HL, Alber T, Thorner J, Nogales E. (2008). Saccharomyces cerevisiae Septins:  Supramolecular organization of hetero-oligomers and the mechanism of filament assembly. Proc. Natl. Acad. Sci. USA. 105, 8274. link

Gay LM, Ng HL, Alber T. (2006). A conserved dimer and global conformational changes in the structure of apo-PknE Ser/Thr protein kinase from Mycobacterium tuberculosisJ. Mol. Biol. 360, 409.

Grundner C, Ng HL, Alber T. (2005). Mycobacterium tuberculosis protein tyrosine phosphatase PtpB structure reveals a diverged fold and a buried active site. Structure 13, 1625.

Pullen KE, Ng HL, Sung PY, Good MC, Smith SM, Alber T. (2004). An alternate conformation and a third metal in PstP/Ppp, the M. tuberculosis PP2C-Family Ser/Thr protein phosphatase. Structure 12, 1947.

Good MC, Greenstein AE, Young TA, Ng HL, Alber T. (2004). Sensor domain of the Mycobacterium tuberculosis receptor Ser/Thr protein kinase, PknD, forms a highly symmetric beta propeller. J. Mol. Biol. 339, 459.

Kopka ML, Han GW, Lavelle L, Ng HL, Dickerson RE. (2003). An unusual sugar conformation in the structure of an RNA/DNA decamer of the polypurine tract may affect recognition by RNase H. J. Mol. Biol. 334, 653.

Ng HL, Dickerson RE. (2002). The mediation of the A<>B DNA transition by guanine tracts. Nucleic Acids Res. 30, 4061.

Dickerson RE, Ng HL. (2001). DNA structure from A to B. Proc. Natl. Acad. Sci. USA 98, 6986.

Ng HL, Dickerson RE. (2001). Mildly eccentric ‘E-DNA’. Nature Struct. Biol. 8, 107.

Šponer J, Florián J, Ng HL, Šponer JE Špacková, N. (2000). Local conformational variations observed in B-DNA crystals do not improve base stacking: computational analysis of base stacking in a d(CATGGGCCCATG)2 BA intermediate crystal structure. Nucleic Acids Res. 28, 4893. 

Ng HL, Kopka ML, Dickerson RE. (2000). The structure of a stable intermediate in the AB DNA transition. Proc. Natl. Acad. Sci. USA 97, 2035.

Marcotte E, Pellegrini M, Ng HL, Rice DW, Yeates T, Eisenberg D. (1999). Detecting protein function and protein-protein interactions from genome sequences. Science 285, 751.

Goodsell D, Ng HL, Kopka ML, Lown JW, Dickerson RE (1995). Structure of a dicationic monoimidazole lexitropsin bound to DNA. Biochemistry 34, 16654.