The sequencing reaction takes place on a flow cell. The MiSeq flow cell consists of one lane. The lane can contain 1 or more samples (see multiplexing) depending the how much coverage is needed for each sample.
For low diversity libraries, we recommend a PhiX spike-in to increase diversity. A typical spike-in is 1% - 5% of the library.
Library Generation prices are dependent on library type, number of samples, and individual project needs. Prices include library generation and library QC. Please contact the Core lab manager for an estimate.
* Includes cycles for both read 1 and read 2 (if applicable).
**Prices subject to change. Cost for quality control assays not included.
Depending on the library generation technique used, anywhere from 2 to 384 individual libraries can be pooled in one lane. Please contact the Core Facility Manager for details.