Project Summary
 
Analyses of 2D Nuclear Magnetic Resonance (NMR) Spectra of CDT

Sepsis is a major health problem that causes substantial mortality across the globe. To combat sepsis, drugs composed of peptides with antibacterial properties (antimicrobial peptides) are crucial. Antimicrobial peptides destroy mirobials by disrupting their outermembrane, which composes mainly of lippopolysaccharide (LPS).

 

One of such antimicrobial peptides that could be potentially used in anti-sepsis drug is CDT, an analogue of Tachyplesin I, extracted from frogs. Experiments on CDT have shown that CDT retain the antimicrobial properties of Tachyplesion I but does not have its hemolytic activity. However, it remains to be known the structure of CDT in its interaction with LPS, which is necessary to understand the implications of structural modifications for the antimicrobial activity of peptides. This knowledge would enable future scientists to synthesize better analogues of Tachyplesin I with improved antimicrobial activity and cell selectivity by modifying its structure and therefore design more effective drugs against sepsis.

 

In this project, I used the software SPARKY to analyze the two-dimenstional Nuclear Magnetic Resonance spectra of CDT-TOCSY (total correlation spectroscopy) and NOESY  (nuclear overhauser enhancement spectroscopy), in its solution state complex with LPS. A technique called sequence specific resonance assignment was used to carry out the analysis. Then I used the results for some structural calculations which showed that the structure of CDT is alpha-helix.


Analyses of 2d-nmr spectra 1of cdt

https://docs.google.com/leaf?id=0B5h36SQYJJ41MDMyMzA3NWUtNWUwYS00MjQ1LThjYzktZjRkMjRiM2I0N2Yw&hl=en