Project Summary

    Cases of prostate cancer, both in early and advanced forms are rising from the already high 217,000 cases in the U.S. alone. In light of these cases nearing a 15% fatality rate, this research project employed gold nanoparticles in colloidal form for the treatment of prostate cancer cells. The experiment took a varied approach in determining at what doses optimal treatment occurred.

    Gold colloid at increasing doses was applied to wells on a tissue culture dish. Each well contained 50,000 prostate cancer cells at the start of the experiment. After 18 to 24 hours in an incubator, the gold colloid was aspirated, and the cells were lifted using the enzyme Trypsin. The data displayed an inverse correlation between the amount of gold colloid and number of cells remaining after treatment. As the amount of gold colloid increased, so did the number of cells killed. This trend remained consistent for each data point. The wells with no treatment condition produced an average of 71,333 prostate cancer cells after the research. At 300 microliters of gold colloid (highest dosage), the number of cancer cells remaining was only 8,250.

    The theory behind the cell's apoptosis is their loss of cell membrane asymmetry leading to PCD through nuclear collapse. This study successfully suggested that gold colloid is a viable prospect for treatment for prostate cancer. The data from the experiment validates the hypotheses. In the future, more dosages with repeated trials could be tested to determine the pharmacology and toxicology of gold nanoparticles.





 

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