When is Gel Electrophoresis used in everyday life?

 Gel electrophoresis is used in everyday life; some examples are:

1. to get DNA fingerprints for forensics

DNA fingerprints can be found using gel electrophoresis; this is possible because when the DNA is placed in the agarose gel or polyacrylamide gel the DNA will move towards the positive end, when the electrophoresis is done the DNA will appear as bands in the gel. This array of bands forms the pattern that is a fingerprint. To find the human fingerprint there is one more step, the DNA from the gel is transferred to a paper called nitrocellulose and then short, radioactive pieces of DNA called probes. These probes bind with specific regions of the DNA, finally when the nitrocellulose is exposed to X-ray film, the regions of DNA that bound to the probe will appear and that will reveal the fingerprint of an individual and that is how gel electrophoresis is used in forensics.

2. Disease Diagnosis

The process in which a scientist can determine what disease you may have is by first grabbing sample from patients, then isolate the germ and also isolate the DNA from the bacteria. PCR (Polymerase chain reaction) the DNA with priers specific for the gene specific to this disease and then after the PCR use gel electrophoresis of the amplified data. After the gel electrophoresis stain the gel and observe the gel through the UV transilluminator and if the band of the amplified DNA matches the band of your control DNA then the patient has the disease.


Comments