Tutorials‎ > ‎

Micropipettors

Micropipettors 


A micropipettor is an instrument used to accurately measure liquid volumes.  We will use Rainin micropipettors for volumes at or below 5 mL, usually in the microliter (μL, 10-6 L) range.  Pipettors are accurate devices.  A p1000 can measure 1 mL volume to a precision of 1.3 μL. 

To set pipetting volume:

  1. Twist the volume lock to loosen (1/8th turn)
  2. Twist the plunger button clockwise or counter-clockwise until the numbers read the proper volume. Note: In between the bottom numbers are four ticks which represent 0.2 divisions.
  3. Lock the volume lock ring.

To transfer liquid:

The plunger button may be depressed at two different stops.  The first is an accurate measure of the total volume.  The second (100% depressed) is a “blow out”.

  1. Put a tip on the pipettor shaft by gently, firmly inserting the shaft into a tip held in the rack.
  2. Depress the plunger button to the FIRST STOP.
  3. Immerse the tip into your liquid a few mm’s and release the plunger slowly.
  4. Dispense the liquid into its designated container slowly by pressing the plunger to the first stop again.
  5. Remove the tip from the liquid and completely press the plunger to the SECOND stop to blow out the remaining drops.
  6. Eject the tip with the white button below the volume lock ring into a beaker or other container designated for tip disposal.

Helpful Notes:

  • ALWAYS PUT ON TIPS BEFORE SUCKING UP YOUR SOLUTION PIPETTE VERTICALLY, DO NOT TILT THE PIPETTOR, NEVER TURN THE PIPETTE UPSIDE DOWN. (This lets liquid into the pipette shaft, which ruins it.)
  • Never try to pipette solutions beyond the limit volume (minimum is 10% of the maximum).
  • It is important to use the right sized tips for the pipettor--do not jam the shaft to make it fit.
  • Use filtered tips when you are starting out.
  • When transferring a small amount of solution (e.g.: using a p20 to transfer 5 μL to a sample vial) to make a solution, it is hard to see if all 5 μL is properly transferred (any volume less than 50 μL would be hard to see).  It is better to have certain amount of the solvent (e.g.: water) in the vial first, and immerse the tip and deliver the 5 μL into the solvent, instead of into an empty vial.  You may also suck up some amount of the solution after delivery and eject again to ensure no residue is left behind on the side wall of the tip.

 

Name

Range

p20

2~20 μL

p200

20~200 μL

p1000

100~1000 μL

p5000

500~5000 μL

 

Comments