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RM 601 Research Methodologies

Title Schedule Download supplementary content Supplementary information/notes/links
Microscopy26/6/2010Presentation

Recommended reading
Nature reviews: Milestones in light microscopy

Spectroscopy

19/7/2010
23/9/2010
30/9/2010
PresentationMeasuring Molar Absorption Coefficient of Proteins (Link to PDF)

In reply to Seema's Q, in practice maximum A value is 3 while it may be possible to get more than this if you increase sensitivity of the instrument. See this image.
 
 

In reply to Mohd. Jahid's Q, Calorimetry and Coulometry (Coulo from Coulombs, unit of electric charge) are quite different. In the former, heat measurements are taken to analyse chemical reactions; while in the latter as well as in a related analytical method electrogravimetry, potentiometric measurement are made in the absence of external current flow. Purdue university's this classroom note explains coulometry very well. Also don't get confused with Colorimetry, measuring color as in  spectrophotometer.

Nucleic Acid Chemistry 1: Basics 22/7/2010Presentation



        

Nucleic Acids Chemistry 2: Extraction 29/7/2010 PresentationCTAB DNA Extraction Protocol
SDS Protocol
Nucleic Acids Chemistry 3:
Detection <Gel Electrophoresis>
 2/12/2010 Presentation 
 Centrifugation 2/12/2010 Presentation 
 Milestones in DNA Technologies  Presentation     


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Felix Bast,
Aug 1, 2010, 9:08 PM
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Felix Bast,
Aug 1, 2010, 9:32 PM
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Felix Bast,
Aug 1, 2010, 10:40 PM
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Felix Bast,
Aug 1, 2010, 9:13 PM
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Felix Bast,
Jun 28, 2010, 3:02 AM
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Felix Bast,
Aug 1, 2010, 9:34 PM
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Felix Bast,
Aug 1, 2010, 9:06 PM
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