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T-cell receptor (TCR) repertoire profiling in non-model species using high-throughput sequencing

posted Aug 21, 2018, 4:10 AM by Magdalena Migalska   [ updated Oct 22, 2018, 4:35 AM by Jacek Radwan ]
High-throughput sequencing (HTS) prompted development of novel, refined methods of analyzing immunological repertoires (e.g. immunoglobulins, TCRs). So far, despite a decade of progress, these techniques were mainly used in research involving model species and humans.

Recently, our group published an article in Scientific Reports describing first use of HTS in TCR repertoire sequencing in a non-model mammal (the bank vole, Myodes glareolusI). The article also presents our newly developed software: AmpliTCR and AmpliCDR3. Programs allow detailed quantitative and qualitative description of TCR repertoires without reference sequences. These tools should become particularly useful in the fields of comparative immunology, ecology and evolutionary biology, which often focus on non-model species.