A gene expression study of how ocean acidification would affect an abundant and widely-distributed calcifying marine phytoplankton species, Emiliania huxleyi. Cells are cultured in continuous-flow chemostats at present day CO2 concentrations (380 ppm) and at concentrations expected at the end of the century (1000 ppm). We have cultured the cells under these conditions for over 250 generations to date and are aiming for 600 generations before gene expression research is done. In addition to CO2 research we are examining growth of E. huxleyi on nutrient limiting concentrations of either ammonium or nitrate.
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