Gru, formerly a super-villain, is now no longer a villain after having saved his three adopted daughters Margo, Edith, and Agnes from rival super-villain Vector in the first film Despicable Me. Gru is having a birthday party for Agnes, the youngest child, in which he impersonates a Fairy Princess to save her from disappointment when the performer cannot attend. Much to Gru’s chagrin, his neighbor, Jillian, begins trying to get Gru to take some of her single acquaintances on a date. While he is outside, he and two of his minions are approached and kidnapped by Lucy Wilde, an Anti-Villain League agent, and taken to the underwater Anti-Villain League Headquarters, where they ask for Gru to become a “spy” in order to track down a new super-villain who has stolen a research laboratory and a chemical compound that can transform lifeforms into invincible mutant killing machines. Gru refuses, saying that he has new responsibilities now. Before he leaves, Lucy admits she was impressed by his work as a villain, stating that stealing the Moon was “amazing”, before giving him her phone number and urging him to contact her if his mind changes.

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