Past Workshops‎ > ‎

2nd ASEAN Workshop

"Flow Cytometry Techniques"
Bioinformatics Institute and Biopolis Shared Facilities,
Agency for Science Technology & Research (A*STAR)
and National University
January 17-21, 2011

The 2nd ASEAN (Association of South East Asian Nations) was hosted by Dr. Vivek Tanavde of the Bioinformatics Institute. Financial support for faculty travel was provided by grants from the International Society for Advancement of Cytometry (ISAC), International Society for Clinical Cytometry (ICCS) and the Bioinformatics Institute, Singapore.

A two day course on basic flow cytometry for students unfamiliar with the technology was taught by Drs. Michael Ormerod and H. Krishnamurthy. Out of 45 applicants, 31 were selected for wet labs where the students prepared samples and analyzed them on the nine flow cytometers/sorters available for the course. More than 50 students from local institutions were present for the lectures.

Wet labs for sample preparation and flow cytometric analysis were conducted at the Biopolis Shared Facilities, National University of Singapore and the Becton Dickinson Training Center. The 13 faculty members (11 from abroad) were supported by 12 staff associates and technical staff from the host institutions and several major flow cytometer manufacturers. Instruments from Becton Dickinson, Beckman Coulter, Millipore, Life Technologies and Miltyeni were used for wet labs. Software support was provided by Tree Star (Flow Jo).

Lab modules taught were: 1. Cell Cycle and Proliferation (Krishan, Cram and Hamelik), Apoptosis (Ormerod), Multicolor Phenotype Analysis (Jain and Maciorowski), Stem cell Analysis (Telford, Krishan), CD34 enumeration (Sutherland), Cell sorting (Osborne). Leukemia & Lymphoma (Gujral), Infectious diseases (Pattanpanyasat) and Cell Signaling (Shankey).

Students were asked to rate the lab modules and lectures as well as to make comments on the workshop. On a scale of poor (1) to outstanding (5), the lectures and labs were rated between very good++ to outstanding with an average score of 3.8.

Out of the 24 respondents, 6 rated the workshop as "exceeded expectations" and 16 indicated that it "met most expectations".

A total of 15 new applications for ISAC membership were collected and submitted to ISAC.

2nd ASEAN Workshop