Dr. Tanavde, V.

Vivek Tanavde obtained his Ph.D from the Cancer Research Institute, Mumbai (1999) in Applied Biology. From 1999 to 2002, he was a post doctoral fellow at the Sidney Kimmel Cancer Centre, Johns Hopkins University working on expansion of hematopoietic stem cells. He returned to India in 2002 and headed the Hematopoietic Stem Cell Lab at Reliance Life Sciences, Mumbai. His laboratory also provided diagnostic services using flow cytometry, mainly in the areas of CD4/CD8 enumeration & single platform CD34 enumeration. In 2006, he joined the Bioinformatics Institute, Singapore as a Research Scientist in the Genome & Gene Expression Data Analysis Division, where he is currently a Principal Investigator. Most of his work has focused on developing protocols for expansion and targeted differentiation of mesenchymal stromal cells. His current research focuses on the use of bioinformatics and systems biology to understand mesenchymal stromal cell biology and differentiation.
Lab Module
CD34 enumeration is a useful tool to enumerate the numbers of hematopoietic stem cells in bone marrow, cord blood or mobilized peripheral blood grafts used for transplantation. CD34+ cells are transplantable hematopoietic stem/progenitor cells found in bone marrow, umbilical cord blood and mobilized peripheral blood. This lab will demonstrate the ISHAGE protocol for enumerating viable CD34+ cells from these tissues using a single platform. In addition issues with sample preparation, quality control and the application of this approach for rare event determination in other cell types will also be discussed.

Relevant Literature

V Tanavde, M Malehorn, R Lumkul, Z Gao, J Wingard, E Garrett & Civin CI. (2002) Human stem-progenitor cells from neonatal cord blood have greater hematopoietic expansion capacity than those from mobilized adult blood. Exp. Hematol 30 pp:816-823.

Keeney M, Chin-Yee I, Weir K, et al. Single platform flow cytometric absolute CD34+ cell counts based on the ISHAGE guidelines. Cytometry 1998;34:61-70.

Keeney M, Sutherland R. Hematopoietic Stem Cells: Issues in Enumeration. In: Applications of Flow Cytometry in 'Stem Cell Research and Tissue Regeneration', Eds. Awtar Krishan, H Krishnamurthy and Satish Totey, Wiley- Blackwell Publications. 2010.