Dr. Jain, P.

Paresh Jain obtained M.D. from the University of Bombay, India (1997) and trained in hemato-oncopathology at the All India Institute for Medical Sciences, New Delhi (1997-2000) and at The Royal Marsden NHS Trust Hospital, London (2000-2001). He worked as Assistant Professor at AIIMS, New Delhi (2001-2005) where he made significant contributions in developing the diagnostic flow cytometry lab and standardizing panels and protocols for flow cytometric characterization of leukemias and minimal residual disease in CLL. Subsequently, he joined BD Biosciences, India as Scientific Advisor (2005 till date) where he has played an active role in institutionalizing CD4 GLP training programs for NACO sites, giving shape and direction to structured training courses in flow cytometry (where more than 500 scholars have received hands-on training on flow cytometry during 2005-2011) and establishing several BD Centers of Excellence for education and training in flow cytometry. Since December 2010, his role and responsibilities have expanded to provide support on Clinical Reagents and Bioimaging products in BD Asia-Pacific Region. He is Executive Committee member of The Cytometry Society-India (TCS) and is responsible for coordinating workshops and training programs.
Lab: "Multicolor Immunophenotyping"
Faculty: Dr Paresh Jain, Dr Amitav Mohanty

Designing and performing a multicolor immunophenotyping experiment requires a sound understanding of instrument, reagent, sample processing, and data interpretation related variables. In this lab, we will illustrate the importance of these variables through a mix of wet labs and tutorials. While the tutorials will cover grammar and vocabulary of building a multicolor panel and experiment, the practical session will focus on instrument setup, compensation and FMO controls using a 6-8 color experiment designed to characterize circulating naïve, memory and regulatory CD4 T-cells in human peripheral blood.

There are four essential variables that can impact flow cytometric analysis namely, instruments, reagents, sample processing and data interpretation. The aim of this lecture is to explain various components of "instrument related variables" in a flow cytometer and discuss conventional and emerging concepts in characterising instrument performance and tracking it over a period of time to ensure data accuracy and reproducibility.

Relevant Literature
Selecting Reagent for Multicolor Applications. Maecker H and Trotter J. Application Note (June 2012): BD Biosciences, San Jose.

Qr and Br in BD FACSDivaT Software: Parameters for Characterizing Detector Performance. Alan Stall. Application Note (June 2012) : BD Biosciences, San Jose