Dr. Narayanan, P.

Padma-Kumar "Padma" Narayanan obtained a BVSc ( College of Veterinary & Animal Sciences, Kerala); a Masters' in Veterinary Surgery (Madras Veterinary College, Tamil Nadu) and a Ph.D in Immunopharmacology from School of Veterinary Medicine, Purdue University, Indiana, USA. His research has focused on understanding the role of reactive oxygen intermediates and mitochondrial dysfunction in cellular patho-physiology, particularly in reticulo-endothelial cells. He utilized analytical cytology tools, to gain insights into mechanisms of neutrophil and endothelial patho-physiology and the role of oxidative stress in radiation-induced DNA damage, cell cycle regulation, silicosis and chronic beryllium disease during his post-doctoral training at Purdue University and at the Los Alamos National Laboratories, Los Alamos, NM. During his ~16-year tenure in the pharmaceutical industry (SmithKline Beecham/GlaxoSmithKline and Amgen, Inc.), he has successfully integrated cytometric technologies and cellular patho-physiological endpoints for identification and characterization of drug-induced pharmacologic/toxicologic responses. He is currently Director of Cell Signaling and Immunotoxicology Group of Discovery Toxicology, Amgen, Inc, Seattle, WA.
Lab Module
We will familiarize students with flow cytometric methods for detection of mitochondrial membrane potential, intracellular glutathione content, mitochondrial superoxide generation and viability in HL-60 cells. We will also isolate neutrophils for monitoring of altered oxidative burst, activity found in various disorders and to evaluate drug effects.

Relevant Literature
Carter WO, Narayanan PK, Robinson JP. Intracellular hydrogen peroxide and superoxide anion detection in endothelial cells. J Leukoc Biol. 1994 Feb;55(2):253-8. J Leukoc Biol. 1994 Feb; 55(2):253-8.

Robinson, J.P., Narayanan, P.K. and Carter, W.O. (1994) Functional Measurements Using HL-60 Cells. In: Methods in Cell Biology: Flow Cytometry, Edited by Darzynkiewicz, Z., Robinson, J.P. and Crissman, H.A. New York: Academic Press. Chapter 25, Vol 42:423.

Robinson, J.P. Carter, W.O. and Narayanan, P.K. (1994) Oxidative product formation analysis by flow cytometry. In: Methods in Cell Biology: Flow Cytometry, Edited by Darzynkiewicz, Z., Robinson, J.P. and Crissman, H.A. New York: Academic Press. Chapter 28, Vol 41:437.

P K Narayanan, P J Bugelski, T K Hart, D M Williams, C Zhang, D. McFarland, L. Hahn, F J Elcock, L W Schwartz, and D G Morgan. (2003) Troglitazone-Induced Intracellular Oxidative Stress In Rat Hepatoma Cells And Primary Rat Hepatocytes: A Flow Cytometric Assessment. Cytometry 52A (1):28-35.