Dr. Kapinsky, M.

Michael Kapinsky started his academic education in classical chemistry before he redirected his scientific ambitions towards life sciences. After a short excursion to plant biochemistry he spent a 4 years-spanning research period in the field of atherosclerosis and received a PhD from Regensburg University in 2002. During his experimental work Michael utilized flow cytometry to characterize an in-vitro model of macrophage-derived foam cell formation and to analyze the related functional significance of cholesterol-enriched membrane raft structures and localized receptors. In 2002 he joined Beckman Coulter's flow cytometry division as an applications specialist with responsibilities in Germany and Switzerland. Beginning of 2012 Michael assumed a role as applications specialist in the global flow cytometry team at Beckman Coulter.
Lab Module
Essential principles of multicolor panel design will be presented in brief and reflected by varying fluorochrome combinations for identical sets of antigens. We will start by pairs of labelled antibodies to elucidate each of the rules step by step and move on to 10 color examples to learn how the rules complement one another. There will be an optional learning assessment in the end allowing all participants to evaluate their knowledge in multicolor panel design.

Relevant Literature
Roederer M, Spectral compensation for flow cytometry: visualization artifacts, limitations, and caveats. Cytometry 45(3):194-205, 2001

Shapiro HM, 5.4 Compensating Without Decompensating. Practical Flow Cytometry 4th ed., 2003

Maecker HT, Frey T, Nomura LE and Trotter J, Selecting Fluorochrome Conjugates for Maximum Sensitivity. Cytometry Part A 62A:169-173, 2004

McLaughlin BE, Baumgarth N, Bigos M, Roederer M, De Rosa SC, Altman JD, Nixon DF, Ottinger J, Oxford C, Evans TG and Asmuth DM, Nine-Color Flow Cytometry for Accurate Measurement of T Cell Subsets and Cytokine Responses. Part I: Panel Design by an Empiric Approach. Cytometry Part A 73A: 400-410, 2008