Dr. Pattanpanyasat, K.

Kovit Pattanapanyasat received his B.Sc and MSc in Biochemistry, (1974, 1977) from the Chulalongkorn University, Bangkok, Thailand. He received a Dip. Bio. Med. Method, (1985) and a Ph.D. in Haematology (1987) from the University of Wales College of Medicine, Cardiff, U.K. He is now Chief, Office for Research and evelopment, and Professor in the Faculty of Medicine, Siriraj Hospital, Bangkok, Thailand.
Wet Lab: Flow Cytometry and Malaria Study
Malaria remains one of the world's major health problem particularly in tropical countries. Enumeration of parasitemia is an important tool to evaluate and follow up disease. Various methods including microscopic counting have been described for detection of malaria parasites in red blood cells (RBCs) in the culture. Because RBCs do not contain DNA, DNA fluorescence from infected RBCs can easily be distinguished from non-infected RBCs, which is proportionally related to the development of the parasites. Therefore, flow cytometry method using nucleic acid dyes has become a method of choice for detection of infected RBCs. They provide higher throughput, statistical power and precision than microscopic determination of blood smears. Apart from parasitemia determination, flow cytometry can also be used to study host-parasite interrelationship in malaria pathogenesis. In particular, changes in RBC surface molecules such as phospholipid asymmetry and knob antigens have provided important insights on alteration of RBC membrane lipids and proteins in association with invasion and maturation of parasites. Simultaneous analysis of parasite DNA, antigens and RBC membrane determinants by flow cytometry has important implications for malaria vaccine development and for understanding malaria pathophysiology.

Relevant Literature
Pattanapanyasat K, Yongvanitchit K, Tongtawe P, Tachavanich K, Wanachiwanawin W, Fucharoen S, Walsh DS. Impairment of Plasmodium falciparum growth in thalassemic red blood cells: Further evidence by using biotin labeling and flow cytometry. Blood 1999;93:3116-3119.

Pattanapanyasat K, Walsh DS, Yongvanitchit K, Piyawatthanasakul N, Wanachiwanawin W, Webster HK. (2003) Robust in vitro replication of Plasmodium falciparum in glycosyl-phosphatidylinositol-anchored membrane glycoprotein-deficient red blood cells. Am J Trop Med Hyg 69: 360-365.

Bhakdi SC, Sratongno P, Chimma P, Rungruang T, Chuncharunee A, Neumann HPH, Malasit P, Pattanapanyasat K. Re-evaluating acridine orange for rapid flow cytometric enumeration of parasitemia in malaria-infected rodents. Cytometry Part A, 2007:71A:662-667.

Pattanapanyasat K, Sratongno P, Chimma P, Polsrila K, Chotivanich K. Febrile temperature but not pro-inflammatory cytokines promotes phosphatidylserine expression on Plasmodium falciparum malaria-infected red blood cells during parasite maturation. Cytometry Part A 2010;77A:515-523.