Alan-Saluk

Alan Saluk obtained a B.S. in Biology from the University of Florida (1992) and did post-baccalaureate studies from Barry University and the University of Miami (Miami, FL).   From 1989 to 1998, he worked at the Coulter Corporation in the Applied Research Division in a wide range of biochemistry oriented projects.  He moved to the Scripps Research Institute in 1998 as a Research Associate and became Director of the Flow Cytometry Core Facility in 2001 to present.  The facility is the largest flow cytometry core facility on the US West Coast and performs the full spectrum of research applications in flow cytometry and high-speed cell sorting.

            Mr. Saluk has been involved with educations initiatives in conjunction with ISAC over the past several years specializing in introductory education as well as core facility management.



 


 

Lecture: Basics of Flow Cytometry.  The basics of flow Cytometry will be discussed in detail.  Lecture sections will include: Basic Concepts in flow cytometry, Applications specific to flow Ccytometry in Biomedical research, limitations to current flow cytometry instrumentation and techniques, and Emerging technologies that address these limitations as well as future trends in cytometric research.

 

Lab: Polychromatic Flow Cytometry: Compensation & FMO.  A novel concept to design multicolor panels will be presented and practiced in this wt lab.  Derived from physical facts e.g. fluorochrome brightness and spectra as well as through consideration of biological features such as antigen expression characteristics and patterns, a set of 6 easily applicable rules will be stepwise developed, discussed and verified through detection of most delicate populations in real samples.

 

Lab: Electrostatic Cell Sorting.  In this wet lab, we will discuss the ins and outs of using electrostatic flow sorting for collection of cells of interest.  Emphasis will be put on quality control, sterility and validation of sorting parameters.  We will have cell sorters from BD, BC and BioRad available for this lab.

 

Lecture: Data Analysis and Display.  In this lecture we will cover concepts and methodologies related to analyzing the data generated by flow cytometric analyses.  Topics addressed will include choosing appropriate statistical methods.

 

Relevant Literature:

 

1.     Maecker, H.T., Frey, T., Nomura, L.E. & Trotter, J. Selecting fluorochrome conjugates for maximum sensitivity. Cytomety A 62, 169–173 (2004). 

2.     Baumgarth, N. & Roederer, M. A practical approach to multicolor flow cytometry for immunophenotyping. J. Immunol. Methods 243, 77–97 (2000)

3.     Leonore A Herzenberg, James Tung, Wayne A Moore, Leonard A Herzenberg & David R Parks  Interpreting flow cytometry data: a guide for the perplexed.   Nature Immunology 7, 681 - 685 (2006)