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In Vitro fertilisation could be the technique of fertilising eggs outside the body. It is commonly attempted in cases of infertility. In this technique, the eggs from a female are taken by way of a needle and incubated with sperms from her partner or perhaps a donor. This results in fertilisation and the fertilised egg is transferred back again to the uterus to grow.

What're the mandatory requirements for IVF?

IVF requires three basic necessities, a wholesome egg, a wholesome sperm and healthy uterus that may carry the pregnancy. This question is necessary because it's not necessary to own all three to produce a bay. Let's like consider women who cannot produce eggs, but has a functional uterus. In this instance egg donation is just a reasonable option. Similarly if sperms of the male partner are defective then donor sperms can also be used. This is called surrogacy. There is another kind of surrogacy; many women end up having their uterus which prevents them from carrying a pregnancy. The embryos made by fertilisation of their eggs using their partner's sperms could be transferred to another woman who has functioning uterus. hcg

Is it possible to describe the procedure?

You will first be given a program of hormonal drugs to enhance your ovaries to produce more follicles (follicles contain eggs). These hormones are mostly FSH analogues and they stimulate the ovaries to produce between 50-60 such follicles. The growth of the follicles will be monitored closely by testing for hormones and serial ultrasound scans. On round the 10th day an attempt of inject able HCG is given that causes these follicles to mature further. These follicles are then aspirated by a needle passed through the vagina and around 30 of the follicles will be aspirated at one time. It can take about 20 min to accomplish this procedure and during this time women will either be under sedation or general anaesthesia. hcg shots

Once these follicles are aspirated, they are delivered to the lab where they will be processed. The eggs are cleaned of their surrounding cells and the very best eggs for fertilisation are selected. Then they mixed with washed sperms (sperms which are removed of most inactive cells and seminal fluid) in a petridish and incubated at 37 degree Celsius for 18 hours. During this time period the fertilised egg may have two pronuclei.In cases when the sperm has difficulty penetrating the ovum, it may be directly injected into the egg by an activity called Intra-cytoplasmic Sperm Injection.Once the eggs are fertilised they are transferred to a culture chamber where they grow for another 3-5 days before being implanted in the female. These embryos may be cultured in artificial culture media or endometrial co-culture. In endometrial coculture the embryo is cultured on cells from woman's own endometrium.hcg shots

Whenever you want 3-5 embryos are positioned in the uterus for implantation based upon many factors.

This was a short summary about the procedure. You can read the whole article in In Vitro Fertilisation like the success rates, complications and other issues associated with this specific procedure from my website.