Cellulophaga lytica Genome Project

Joanna R. Klein
Northwestern College, St. Paul, MN

Research Abstract

Cellulophaga lytica, is a marine bacterium that was recognized as a novel species in 1999 and represents a poorly understood branch of the tree of life.   Bacteria closely related to C. lytica often have the ability to degrade polysaccharides such as cellulose, the main component of plant cell walls.  The use of cellulose containing materials in the production of ethanol is an active area of research with the goal of reducing our reliance on fossil fuels and addressing current limitations of producing ethanol from corn.   Therefore, cellulose degrading bacteria are of particular interest as researchers study the role microorganisms have in energy production.  The Joint Genome Institute (JGI) of the Department of Energy, has sequenced the entire genome (DNA sequence) of C. lytica.  In collaboration with the JGI, we are identifying and characterizing genes within this organism in a process called bioinformatics and genome annotation.  Additionally, we are examining the physiology and motility of the bacterium in the laboratory.  The goal of our research is to shed light on this poorly understood organism and contribute to bioenergy research and applications.   

Summary of Research Program and Progress

Students in Genetics and Microbiology courses have participated in gene annotation.
Independent research students have done examined growth properties and motility.
  (See student research page)

Annotation Tally
  • Number of open reading frames (ORFs) annotated in this organism:
    • 15 genes fully annotated
    • 10 genes partially annotated
  • The number of ORFs confirmed by functional analysis:
  • Number of biochemical pathways annotated in this organism:
  • Number of partial biochemical pathways annotated in this organism:

Are you interested in joining the Interpret a Genome program?  More information and the opportunity to receive training can be found at the DOE/JGI Interpret a Genome Program and the Microbial Genome Annotation Network (MGAN) websites (See navigation bar). 

This program is a collaboration with the Department of Energy (DOE) Joint Genome Institute (JGI) as part of the Genomic Encyclopedia for Bacteria and Archaea (GEBA) project using the Integrated Microbial Genomes Annotation Collaboration Tool (IMG-ACT).

 The Design of this website template was funded by NSF (RCN-UBE-DBI 0954829)