Week 06: Kinetics and Catalysis

Lecture 11:

Outline

1. Mixed inhibition
   1. General derivation
   2. Limiting cases: "competitive," "uncompetitive," and "noncompetitive"
2. Reversible phosphorylation ("Push-pull" systems)
   1. Single site
   2. Multisite
   3. Zeroth order ultrasensitivity

Reading

(optional) A. Goldbeter and D. E. Koshland, Jr., “An amplified sensitivity arising from covalent modification in biological systems,” Proc. Natl. Acad. Sci. USA, vol. 78, no. 11, pp. 6840–6844, 1981. (pdf)

Slides

(pdf)

Lecture 12: Thermodynamic analysis of reaction mechanisms

Slides

(Reaction thermodynamics - binding and allostery)

(Simple model of avidity)