The following is from our PBworks page as of 05/30/2012:
OBJECTIVE: Hack a cell culture to produce bioluminescent light using luciferase
participants: anitha, cameron, jason, jessabella, kalpith, kim, matheo, michael, patrik d, patrick s, scott, steve
As a start, the pGLO infrastucture from the Green Fluorescence Protein (GFP) Experiments could be used, substituting the GFP gene with the luciferase gene, using the presence of arabinose to start gene expression and therefore luminescent activity. The 2010 E. glowli iGEM project from Cambridge produced a construct that would be an excellent starting point: http://partsregistry.org/Part:BBa_K325909
Further down the road we can think of other ways to activate the bioluminescent behaviour, add our own "bioLOGIC"
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(patrick: this talk inspired and motivated me to start this project. Taking it as a fact that bacteria and cells in general do communicate with the cellular network ouround them and are "aware" of their surroundings, the following questions and ideas arise: How can we "communicate" with cells? What will cultures do when you give them "lifestyle" choices?
So I am interested in creating heterogenous environments for cell cultures, instead of giving them just their desired medium and letting it sit in their desired temperature.
Petri dish with gradient temperature difference (controlled with open source and hardware e.g. with arduino)
Also create experiment environments where cultures can choose/ "grow towards" different:
- Different wavelength of light
- environments with competing culture strains
- absence of liquid / nutrients,)
So to recap: find out their "Likes" and "Dislikes", survival strategies, communication....
If we want to extract the lux operon from Vibrio directly, and make our own plasmid from scratch, ATCC Strain Number 7744 might provide an inexpensive ($40) source of the lux genes. Need to check GenBank.
Bio-light : "The bio-light uses different biological technologies to create ambient light effects. The concept explores the use of bioluminescent bacteria, which are fed with methane and composted material (drawn from the methane digester in the Microbial Home system). Alternatively the cellular light array can be filled with fluorescent proteins that emit different frequencies of light."
Meeting Agenda & Notes 6/3/131. Dino's need careWe need to re-innoculate new cultures which includes larger flasks, more nutrients and filtering out the detritus (can be done simply by removing the ...
Posted Jun 3, 2013, 3:48 PM by Cameron Clarke
Meeting Agenda & Notes 5/20/13We need to get a little more structured for our meetings(thanks, Patrik). We have lots to accomplish so I want to try to streamline our time together. Here's ...
Posted May 21, 2013, 1:20 AM by Cameron Clarke
pbworks content addedPages from the old pbworks site have been added to this google site. Let me know if anything is missing.
Posted May 16, 2013, 4:07 PM by Gavan Wilhite