Research Techniques

Electrophysiology: Patch clamp, Two-electrode voltage clamp (TEVC), iontophoresis, electroretinogram (ERG), muscle mechanics, electromyography (EMG)

Confocal microscopy, Fluorescence microscopy, Calcium imaging, intra-organellar imaging, cytoskeletal imaging, and FRET

Transmission Electron Microscopy

X-Ray Diffraction, Differential Scanning Calorimetry, and thermogravimetric analysis

Proficiency in characterization and development of countermeasures against botulinum neurotoxin intoxication

Mitochondrial oxygen consumption, membrane potential, and calcium transient measurements

Mitochondrial oxygen consumption and cellular oxygen consumption measurements

Cellular oxygen consumption/nitric oxide production measurements 

Intracellular Reactive oxygen/nitrogen species measurements with fluorescent probes

            Cell culture (primary and cell lines) and transfection techniques, stable cell line development

            Reporter assays and cell-based assays for new chemical entity (NCE) drug screening

Cloning, PCR, qRT-PCR

Immunoblotting, coimmunoprecipitation, intracellular cGMP and inositol phosphate (IP) turnover measurements

Site-Directed Mutagenesis

Tissue preparations of mouse extensor digitorum longus, triangularis sterni, diaphragm and rat thoracic aorta rings, and strips

            Drug/pharmaceutical development

            Adipocytes (epididymal, inguinal and brown) and fat pad isolation

Rat/mouse models of obesity, diabetes, adjuvant-induced arthritis and the mouse model for botulinum neurotoxin poisoning, inflammation/pain 

Metabolic activity determination - Oxymax System

Novel Drug Delivery systems: Transdermal, Transmucosal delivery systems, sustained release and controlled release formulations, solid dispersions, solvent evaporation techniques

Nanotechnology - Magnetic nanoparticles for target site-specific drug delivery

Drug dissolution, disintegration, accelerated stability analysis of drug formulations

Targeted drug delivery: Nanosomes, magnetic nanospheres, nanoparticles, nanosuspensions, sustained/controlled formulations for external use, and neuronal drug delivery via cargo proteins         

            Behavioral Pharmacology: Rotarod, Morris water maze, pole climbing, plethysmography, von Frey, Thermal                     plantar, sniffing assay, Hargreaves thermal sensitivity,  Randall Selitto Analgesiometry, thermal preference,      
             mating behavior

            C elegans:
Optogenetics, intracellular calcium and pH measurements in unrestrained worms, Inositol 1,4,5-                     trisphosphate (IP3) receptor function