Asim K. Bepari, PhD (Neuroscience)

I am working in the field of molecular and cellular neuroscience using both in vivo (mouse, chick) and cell culture models. Previously I obtained my bachelor and masters degrees in the field of pharmaceutical sciences. I am aiming my future research in understanding pathophysiology of human diseases, and in the long run, finding better therapeutic interventions.

Research Skills:

v Common techniques of molecular biology and genetics

v Histology: In situ hybridization (Standard, Fluorescence), Immunohistochemistry

v Mouse behavioral tests: Handedness, Avoidance test, Mating assay, etc.

v In ovo electroporation

v Cell culture, Primary neuronal culture, Slice culture

v Epigenetics: Analysis of DNA methylation using bisulfite sequencing

Academic Credentials:

PhD (Neuroscience): March 2013, Kumamoto University, Kumamoto, Japan

Master of Pharmacy (M.Pharm): 2002, Jahangirnagar University, Dhaka, Bangladesh

Bachelor of Pharmacy (B.Pharm): 2001, Jahangirnagar University, Dhaka, Bangladesh

Graduate Research Projects:

1. Mapping brain activity by in situ hybridization of immediate early genes (IEG):

The study involved in vivo olfactory stimulation in mice using synthetic or biological odors and behavioral tests followed by histological analyses. We observed strong IEG induction in the olfactory circuit.

2. Identification of photoactivated neurons by IEG in situ hybridization:

Optogenetic stimulation was done in the striatum of awake mice expressing a variant of channelrhodopsin, ChR2(C128S). Induction of some common IEGs like c-fos and Arc was not significant, whereas, another activity dependent gene Npas4 showed specific induction.

3. Brain lateralization in mice:

In a line of transgenic mice, the transgene expression was found to show significant left-right asymmetry in the hippocampus. This asymmetry was unrelated to handedness but showed relation to the status of DNA methylation.

Awards:

·         ‘Financial support for young scientists’, Global COE, Kumamoto University, Japan, 2011

·         GCOE Travel Award for Young Scientists, Global COE, Kumamoto University, Japan, 2011

·         Global COE Jr. Research Associate, Global COE, Kumamoto University, Japan, 2011

·         Monbukagakusho Fellowship for doctoral study, MEXT, Japan, 2009-2013

Employment History:

·  July 2013- to date: Assistant Professor, Department of Pharmaceutical Sciences, North South University, Dhaka, Bangladesh.

· January 2007-June 2013: Lecturer, Department of Pharmaceutical Sciences, North South University, Dhaka, Bangladesh.

·  April 2013 - June 2013: Researcher, Division of Neurobiology and Anatomy, Graduate School of Medical and Dental Sciences, Niigata University, Japan

· September 2004 - December 2006: Lecturer, Department of Pharmacy, Stamford University Bangladesh, Dhaka, Bangladesh.

List of Publications

Links: Google scholar, ORCiD ID: 0000-0001-5656-1833, ResearcherID: D-4001-2013

Original research articles:

1.      Bepari, A.K., Sano, H., Tamamaki, N., Nambu, A., Tanaka, K.F., and Takebayashi, H. (2012). Identification of optogenetically activated striatal medium spiny neurons by Npas4 expression. PLoS ONE 7: e52783. [Pubmed]

2.      Bepari, A.K., Watanabe, K., Yamaguchi, M., Tamamaki, N, and Takebayashi, H. (2012). Visualization of Odor-induced Neuronal Activity by Immediate Early Gene Expression. BMC Neuroscience 13: 140. [Pubmed]

3.      Watanabe, K., Takebayashi, H., Bepari, A.K., Esumi, S., Yanagawa, Y., Tamamaki, N. (2011). Dpy19L1, a multi-transmembrane protein, regulates radial migration of glutamatergic neurons in the developing cerebral cortex. Development 138: 4979-4990. [Pubmed]

4.      Bepari, A.K., Mazumder, M.E.H., Chowdhury, S.S., and Saha, P. (2007). Evaluation of the Potency of Amoxicillin Trihydrate Active Ingredient Manufactured Locally in Bangladesh. USTA1: 55-59.

5.      Bepari, A.K., Mazumder, M.E.H., Chowdhury, S.S., and Saha, P. (2006).Comparative study of the quality of four amoxicillin capsule brands marketed in Bangladesh in terms of packaging features, potency and dissolution profile. Bangladesh J. Life Sci. 18: 91-96.

Abstracts

1.      Bepari, A.K., Watanabe, K., Yamaguchi, M., Tamamaki, N., and Takebayashi, H. (2012). Sensitive detection of neuronal activity by immediate early gene expression. Journal of Neurochemistry 123, Suppl. 1, 128.

2.      Bepari, A.K., Yamaguchi, M., Tamamaki, N., and Takebayashi, H. (2011). Detection of activity dependent gene expression in olfactory circuit by in situ hybridization probe set. Neuroscience Research 71, Supplement, e153.

3.      Watanabe, K., Bepari, A.K., Takeda, N., Araki, K., and Takebayashi, H. (2012). Roles of Dpy19 family in development of the cerebral cortex. Journal of Neurochemistry 123, Suppl. 1, 49.

4.      Watanabe, K., Takebayashi, H., Bepari, A.K., Esumi, S., Yanagawa, Y., and Tamamaki, N. (2011). Dpy19L1, a multi-transmembrane protein, is required for radial migration of glutamatergic neurons in the developing neocortex. Neuroscience Research 71, Supplement, e230.

Poster presentations

1.      Bepari, A.K., Watanabe, K., Yamaguchi, M., Tamamaki, N, and Takebayashi, H. (2012). Sensitive detection of neuronal activity by immediate early gene expression. The 11th Biennial Meeting of the Asian Pacific Society for Neurochemistry, The 55th Annual Meeting of the Japanese Society for Neurochemistry. Kobe International Conference Center, 6-9-1 Minatojima-nakamachi, Chuo-Ku, Kobe, Japan. 2012.9.28-2012.10.02.

2.      Takebayashi, H., Bepari, A.K., Yamaguchi, M., and Tamamaki, N. (2011). Brain Response to environmental change: Odor-evoked induction of activity-dependent gene expression in mouse brain. 54th Annual Meeting of. The Japanese Society for Neurochemistry. Ishikawa, Japan. P2-11, 2011.9.26-28.

3.      Bepari, A.K., Yamaguchi, M., Tamamaki, N., and Takebayashi, H. (2011). Detection of activity dependent gene expression in olfactory circuit by in situ hybridization probe set. The 34th Annual Meeting of the Japan Neuroscience Society −Neuroscience of the Mind−. Pacifico Yokohama, 1-1-1 Minato Mirai, Nishi-ku, Yokohama 220-0012, Japan. P2-j17, 2011.09.14-17.

4.      Watanabe, K., Takebayashi, H., Bepari, A.K., Esumi, S., Yanagawa, Y., and Tamamaki, N. (2011). Dpy19L1, a multi-transmembrane protein, is required for radial migration of glutamatergic neurons in the developing neocortex. The 34th Annual Meeting of the Japan Neuroscience Society −Neuroscience of the Mind−. Pacifico Yokohama, 1-1-1 Minato Mirai, Nishi-ku, Yokohama 220-0012, Japan. P3-e07, 2011.09.14-17.

5.      Bepari, A.K., Yamaguchi, M., Tamamaki, N., and Takebayashi, H. (2011). Brain response to environmental change: Odor-evoked induction of activity-dependent gene expression in mouse brain. KEY Forum in Developmental Biology and Regenerative Medicine. 100th Anniversary Memorial Hall, Kumamoto University, Kumamoto, Japan. 1-26, 2011.09.8-9.

6.      Watanabe, K., Takebayashi, H., Bepari, A.K., Esumi, S., Yanagawa, Y., and Tamamaki, N. (2010). Novel transmembrane protein Gsg1 is essential for radial migration and dendrite formation of glutamatergic neurons in the Cerebral cortex. THE 29th NAITO CONFERENCE ON GLIA WORLD- Dynamic Function of Glial Cells in the Brain. Shonan Village, Hayama-machi Miura-gun, Kanagawa, Japan. 2010.10.05-08.

7.      Bepari, A.K., Usui, N., Ikenaka, K., Tamamaki, N., and Takebayashi, H. (2010). Left-right brain asymmetry in mice. 2010 Global COE-IMEG Joint Summer Retreat Seminar in Aso. Mt. Aso, Japan.  2010.09.09-10.

8.      Takebayashi, H., Bepari, A.K., Usui, N., Ikenaka, K., and Tamamaki, N. (2010). Analysis on brain left-right asymmetry. Fourth Neural Development Seminar. Okazaki, Japan 2010.03.19-20.