Organic Spectroscopy 



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PAGE CREATED BY DR ANTHONY MELVIN CRASTO M.SC, Ph.D (ORGANIC CHEMISTRY,24+ years experience in the field of research and development, currently with Glenmark-Generics Ltd, Navi Mumbai,  India
Ентоні  アンソニー  Αντώνιος  安东尼    แอนโทนี   Энтони   אַנטאַני  Антхони  एंथनी  안토니  أنتوني


Principal scientist, GLENMARK-GENERICS LTD

Navi mumbai, INDIA

Email  amcrasto@gmail.com


TWITTER-   @amcrasto

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What is Spectroscopy?

Spectroscopy pertains to the dispersion of an object's light into its component colors (i.e. energies). By performing this dissection and analysis of an object's light, astronomers can infer the physical properties of that object (such as temperature, mass, luminosity and composition).

But before we hurtle headlong into the wild and woolly field of spectroscopy, we need to try to answer some seemingly simple questions, such as what is light? Andhow does it behave? These questions may seem simple to you, but they have presented some of the most difficult conceptual challenges in the long history of physics. It has only been in this century, with the creation of quantum mechanics that we have gained a quantitative understanding of how light and atoms work. You see, the questions we pose are not always easy, but to understand and solve them will unlock a new way of looking at our Universe.

The Nature of Light

To understand the processes in astronomy that generate light, we must realize first that light acts like a wave. Light has particle-like properties too, so it's actually quite a twisted beast (which is why it took so many years to figure out). But right now, let's just explore light as a wave.

Picture yourself wading around on an ocean beach for a moment, and watch the many water waves sweeping past you. Waves are disturbances, ripples on the water, and they possess a certain height (amplitude), with a certain number of waves rushing past you every minute (the frequency) and all moving at a characteristic speed across the water (the wave speed). Notice the distance between successive waves? That's called the wavelength.

Keeping this analogy in mind, let's leave the ocean beach for a while and think about light like a wave. The wave speed of a light wave is simply the speed of light, and different wavelengths of light manifest themselves as different colors! The energy of a light wave is inversely-proportional to its wavelength; in other words,low-energy waves have long wavelengths, and high-energy light waves have short wavelengths.

The Electromagnetic Spectrum

Physicists classify light waves by their energies (wavelengths). Labeled in increasing energy, we might draw the entire electromagnetic spectrum as shown in the figure below:

The Electromagnetic Spectrum. Notice how small the visible region of the spectrum is, compared to the entire range of wavelengths.

Notice that radio, TV, and microwave signals are all light waves, they simply lie at wavelengths (energies) that your eye doesn't respond to. On the other end of the scale, beware the high energy UV, x-ray, and gamma-ray photons! Each one carries a lot of energy compared to their visible- and radio-wave brethren. They're the reasons you should wear sunblock, for example.

When we look at the Universe in a different "light", i.e. at "non-visible" wavelengths, we probe different kinds of physical conditions -- and we can see new kinds of objects! For example, high-energy gamma-ray and X-ray telescopes tend to see the most energetic dynamos in the cosmos, such as active galaxies, the remnants from massive dying stars, accretion of matter around black holes, and so forth. Visible light telescopes best probe light produced by stars. Longer-wavelength telescopes best probe dark, cool, obscured structures in the Universe: dusty star-forming regions, dark cold molecular clouds, the primordial radiation emitted by the formation of the Universe shortly after the Big Bang. Only through studying astronomical objects at many different wavelengths are astronomers able to piece together a coherent, comprehensive picture of how the Universe works!

General Types of Spectra

Typically one can observe two distinctive classes of spectra: continous and discrete. For a continuous spectrum, the light is composed of a wide, continuous range of colors (energies). With discrete spectra, one sees only bright or dark lines at very distinct and sharply-defined colors (energies). As we'll discover shortly, discrete spectra with bright lines are called emission spectra, those with dark lines are termed absorption spectra.

Continuous Spectra

Continuous spectra arise from dense gases or solid objects which radiate their heat away through the production of light. Such objects emit light over a broad range of wavelengths, thus the apparent spectrum seems smooth and continuous. Stars emit light in a predominantly (but not completely!) continuous spectrum. Other examples of such objects are incandescent light bulbs, electric cooking stove burners, flames, cooling fire embers and... you. Yes, you, right this minute, are emitting a continuous spectrum -- but the light waves you're emitting are not visible -- they lie at infrared wavelengths (i.e. lower energies, and longer wavelengths than even red light). If you had infrared-sensitive eyes, you could see people by the continuous radiation they emit!

Discrete Spectra

Discrete spectra are the observable result of the physics of atoms. There are two types of discrete spectra, emission (bright line spectra) and absorption (dark line spectra). Let's try to understand where these two types of discrete spectra.

Emission Line Spectra

Unlike a continuous spectrum source, which can have any energy it wants (all you have to do is change the temperature), the electron clouds surrounding the nuclei of atoms can have only very specific energies dictated by quantum mechanics. Each element on the periodic table has its own set of possible energy levels, and with few exceptions the levels are distinct and identifiable. 
Atoms will also tend to settle to the lowest energy level (in spectroscopist's lingo, this is called the ground state). This means that an excited atom in a higher energy level must `dump' some energy. The way an atom `dumps' that energy is by emitting a wave of light with that exact energy.

In the diagram below, a hydrogen atom drops from the 2nd energy level to the 1st, giving off a wave of light with an energy equal to the difference of energy between levels 2 and 1. This energy corresponds to a specific color, or wavelength of light -- and thus we see a bright line at that exact wavelength! ...an emission spectrum is born, as shown below:

An excited Hydrogen atom relaxes from level 2 to level 1, yielding a photon. This results in a bright emission line.

Tiny changes of energy in an atom generate photons with small energies and long wavelengths, such as radio waves! Similarly, large changes of energy in an atom will mean that high-energy, short-wavelength photons (UV, x-ray, gamma-rays) are emitted.

Absorption Line Spectra

On the other hand, what would happen if we tried to reverse this process? That is, what would happen if we fired this special photon back into a ground state atom? That's right, the atom could absorb that `specially-energetic' photon and would become excited, jumping from the ground state to a higher energy level. If a star with a `continuous' spectrum is shining upon an atom, the wavelengths corresponding to possible energy transitions within that atom will be absorbed and therefore an observer will not see them. In this way, a dark-line absorption spectrum is born, as shown below:

A hydrogen atom in the ground state is excited by a photon of exactly the `right' energy needed to send it to level 2, absorbing the photon in the process. This results in a darkabsorption line.

How does a spectrometer work?

Many people know how a telescope works, but relatively few have much experience with the innards of a spectrometer. So let's take apart the Astronomy Camp spectrometer to see how it works! Keep in mind that there are as many optical designs for spectrometers as there are optical designs for telescopes, and that this is but one example. Nevertheless, it points out the salient features of most optical spectrometers.

It all starts with the telescope light beam entering the spectrometer. The focal point of the telescope beam is brought to the slit of the spectrometer. This slit is what is ultimately imaged on the detector. In the case of the Camp spectrometer, the slit is arranged at an angle and the slit surroundings are silvered so that the portion of the telescope beam not passing through the slit can be routed instead to an eyepiece for easy telescope guiding.
The light passing through the slit then is reflected off a collimating mirror, which parallelizes the beam of light, before sending it off...
... to the diffraction grating! This optical element disperses the parallel beams of light into their component colors/wavelengths/energies. Each different wavelength comes off of the grating at a slightly different angle. So now, we have an image of the slit that is spread out like a rainbow by color.
This new color-dispersed beam of light is then focused and imaged on the detector by the camera lens. A 35 mm camera is the detector in this diagram, but at Camp, we typically use an eyepiece or a CCD array.

So, now let's put all of this together to make a spectrometer!

There is something interesting to note here -- in spectroscopy, we are not looking at ALL of the light from an object, just a certain "band" of wavelengths or colors. Furthermore, even that band is dispersed ("smeared out") over the entire detector. This means that the effective brightness, or surface brightness of an object on the detector is much lower than when simply taking images of an object. This means that it takes a bigger telescope and/or more integration time to get a good spectrum of a given object than an image.

The broader you disperse the light and the narrower you make the slit, the better your spectral resolution; you can see finer and more subtle features in the spectrum. However, there is a stiff price to pay: the emergent spectrum becomes much dimmer and more diffuse. High resolution spectroscopy therefore requires large telescopes and fairly bright objects. For very faint objects, some spectral resolution often must be compromised to even SEE the object.

Spectroscopy Sources 6 1 07

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DR ANTHONY CRASTO, PhD, ICT Organic chemistry, Currently working with GLENMARK GENERICS LTD research centre as Principal Scientist, process research (bulk actives) at Mahape, Navi Mumbai, India, helping millions, million hits on google on all organic chemistry websites, Hands on experience in developing novel routes for drug molecules and implementation on commercial scale. several international patents published. amcrasto@gmail.com

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Ph.D from ICT, Mumbai,ORG CHEM, Prof. S. SESHADRI, 25+ years in industry( API), mostly multinationals, process development in companies like Hoechst Marion Roussel(Sanofi aventis), Searle, RPG, Innovassynth, Glenmark, with exposure to basic research at SEARLE under Dr Nagarajan (Director Ciba), Tech transfer in plant eg deltamethrin 3 tonnes in RPG, bulk drug gmp (only 7 months) in Hoechst Marion Roussel(SANOFI AVENTIS), Custom syn in Innovasynth(SANDOZ, Basel, BASF Germany), exposure to natural pdts, drugs int, vet drugs, and some exposure in agro.speciality chemicals, flavours and fragrances, nutraceuticals and mettalocenes. ability to deliver products in the production plant(atleast 35 in career).
  • DR ANTHONY MELVIN CRASTO Ph.D Serving chemists around the world. Helping them with websites on Chemistry.Million hits on google NO ADVERTISEMENTS , ACADEMIC , NON COMMERCIAL SITEs, world acclamation from industry, academia, drug authorities for websites, blogs and educational contribution
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DR ANTHONY MELVIN CRASTO Ph.D , Born in Mumbai in 1964 and graduated from Mumbai University, Completed his  PhD from ICT ,1991,  Mumbai, India in Organic chemistry, The thesis topic was Synthesis of Novel Pyrethroid Analogues, Currently he is working with GLENMARK- GENERICS LTD, Research centre as Principal Scientist, Process Research (bulk actives) at Mahape, Navi Mumbai, India. Prior to joining Glenmark, he worked with major multinationals like Hoechst Marion Roussel, now Sanofi Aventis,  & Searle India ltd, now Rpg lifesciences, etc. He has worked in Basic research, Neutraceuticals, Natural products, Flavors, Fragrances, Pheromones, Vet Drugs, Drugs, formulation, GMP etc. He has total 25 yrs exp in this field, he is now helping millions, has million hits on google on all organic chemistry websites.His New Drug Approvals ,  Green Chemistry International,  Eurekamoments in Organic Chemistry ,   WIX BLOG WORLD DRUG TRACKERare some most read chemistry blogs, He has hands on experience in initiation and developing novel routes for drug molecules and implementation them on commercial scale over a 25 year tenure, good knowledge of IPM, GMP, Regulatory aspects, he has  several international drug patents published worldwide . He has good proficiency in Technology TransferSpectroscopy Stereochemistry Synthesis, Reactions in Org Chem Polymorphism Pharmaceuticals Medicinal chemistry Organic chemistry literature Patent related site Green chemistry Reagents R & D Molecules Heterocyclic chem Sourcing   etc He suffered a paralytic stroke in dec 2007 and is bound to a wheelchair, this seems to have injected feul in him to help chemists around the world, he is more active than before and is pushing boundaries, he has one lakh connections on all networking sites, He makes himself available to all, contact him on  +91 9323115463, amcrasto@gmail.com

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