Cycloserine Cefoxitin Fructose Agar with Horse Blood and Tarocholate (CCFA-HT) is an enriched selective and differential medium for the isolation and presumptive identification of Clostridium difficile, a recognized cause of pseudomembraneous (antimicrobial agent-associated) colitis and Clostridium difficile associated diarrhea (CDAD). The basic nutritive base consists of animal peptones and fructose and is supplemented with cefoxitin and cycloserine at concentrations that inhibit the growth of most normal fecal flora. Horse blood is added as an enriched nutrient source and Taurocholic acid is added as a stimulator of spore germination. Cycloserine will inhibit gram-negative bacteria, while cefoxitin will inhibit both gram-positive and gram-negative organisms. The pH of the media is decreased due to the fermentation of fructose. Most strains of Clostridium difficile are not inhibited on the CCFA-HT medium, and, when growing on this medium, will exhibit a characteristic colonial morphology. This medium is prepared, stored and dispensed under oxygen-free conditions to prevent the formation of oxidized products prior to use.
Anaerobe Systems PRAS Cycloserine Cefoxitin Fructose Agar w/ Horse Blood & Taurocholate
Cycloserine Cefoxitin Fructose Agar W/ Horse Blood and Taurocholate Products