Cycloserine Cefoxitin Fructose Agar (CCFA) is an enriched selective and differential medium for the isolation and presumptive identification of Clostridium difficile, a recognized cause of pseudomembraneous (antimicrobial agent-associated) colitis and Clostridium difficile associated diarrhea (CDAD). The basic nutritive base consists of animal peptones and fructose and is supplemented with cefoxitin and cycloserine at concentrations that inhibit the growth of most normal fecal flora. Cycloserine will inhibit gram-negative bacteria, while cefoxitin will inhibit both gram-positive and gram-negative organisms. Neutral red is added as a pH indicator. The pH of the media is decreased due to the fermentation of fructose, which will turn the indicator from pink/orange to yellow. Most strains of Clostridium difficile are not inhibited on the CCFA medium, and, when growing on this medium, will exhibit a characteristic yellow, ground-glass colonial morphology. This medium is prepared, stored and dispensed under oxygen-free conditions to prevent the formation of oxidized products prior to use.
Anaerobe Systems PRAS Cycloserine Cefoxitin Fructose Agar (CCFA) AS-213