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Tryptic Soy Serum Bacitracin Vancomycin Agar (TSBV)

Tryptic Soy Serum Bacitracin Vancomycin agar (TSBV) is an enriched selective medium for the isolation and presumptive identification of Actinobacillus actinomycetemcomitans. TSBV medium contains bacitracin and vancomycin at a concentration that inhibits most gram-positive and most gram-negative anaerobes, except for Actinobacillus actinomycetemcomitans among others.  This medium is prepared, dispensed and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use. 

     Anaerobe Systems PRAS Tryptic Soy Serum
     Bacitracin Vancomycin Agar (TSBV) AS-648



Tryptic Soy-Serum-Bacitracin-Vancomycin Agar Products
 

 Item #

Description 

Size 

Price 

AS-648

TSBV mono plate 

4 plates 

8.49

 



Formula

Tryptic Soy Agar, 40.0 g

Yeast Extract, 1.0 g

Bacitracin, 75.0 mg

Vancomycin, 5.0 mg

Horse Serum, 100.0 ml

Distilled Water, 1000.0 ml

 

Final pH 7.1 +/- 0.2 at 25 degrees C.

Final weight 16.0 g +/- 1.6.

                                                                                                               

Precautions

For IN VITRO DIAGNOSTIC USE only.  Approved biohazard precautions and aseptic techniques should be observed when using this product.  This product is for use only by properly-trained and qualified personnel.  Sterilize all biohazard waste prior to disposal.

Storage and Shelf Life

Storage:  Upon receipt, store at room temperature (13°C - 27°C) in original container until use.  Avoid overheating or freezing.  Do not use medium if there are signs of deterioration (shrinking, cracking or discoloration due to oxidation of media) or contamination.  The expiration date applies to the product in its original packaging and stored as directed.  Do not use product past the expiration date shown on the container.

Shelf Life:  90 days from date of manufacture.

Procedure

Specimen Collection:  Specimens for anaerobic culture should be protected from air (oxygen) during collection, transport and processing.  Consult appropriate references for detailed instructions concerning collection and transport of anaerobes.Methods for Use:  TSBV should be inoculated directly with clinical material or a broth that has been previously inoculated from clinical material.  Inoculated plates should be streaked to obtain isolated colonies, immediately placed in an anaerobic atmosphere and incubated at 35-37oC for 18 – 48 hours.  Additional periods of incubation may be necessary to recover some anaerobes.  Detailed instructions for processing anaerobic cultures can be found in the appropriate references.

Materials Required But Not Provided

Standard microbiological supplies and equipment such as loops, saline blanks, slides, staining supplies, microscope, incinerator / autoclave, incubators, anaerobic chamber, other culture media and serological and biochemical reagents.

Limitations

TSBV will not provide complete information for identification of bacterial isolates.  Additional test procedures and media are required for complete identification.  It is recommended that a non-selective medium such as Anaerobic Brucella Blood Agar also be inoculated from clinical specimens to assure growth of all species present.  Consult reference materials for additional information.

Quality Control

This medium should support good growth of A. actinomycetemcomitans found in clinical infections.

The following organisms are routinely used for quality assurance performance testing at Anaerobe Systems. 

Organism Tested

ATCC #

Results

Time (Hours)

Actinobacillus actinomycetemcomitans

29522

Growth

24 – 48 h

Actinomyces viscosus

43146

No Growth

 

Fusobacterium nucleatum

25586

Growth

24 – 48 h

Propionibacterum acnes

9619

No Growth

 

Clostridium difficile

9689

No Growth

 

Enterococcus faecalis

29212

No Growth

 

 

User Quality Control: The final determination to the extent and quantity of user laboratory quality control must be determined by the end user.

If sterility testing is to be performed on this product, the appropriate percentage of the original shipment amount should be incubated anaerobically and aerobically for 48 – 96 hours.

If the nutritive/inhibitory capacity of this medium is to be tested for performance, it is recommended that the following ATCC organisms be evaluated for growth/inhibition.

 

Organism

Expected Growth

A. actinomycetemcomitans

24-48 hrs

A. viscosus

Inhibited

F. nucleatum

24-48 hrs

E. faecalis

Inhibited

 

Physical Appearance:  TSBV should appear translucent yellow in color.

 

References

1.        Dowell, V. R., Jr. and T. M. Hawkins.  1974.  Laboratory Methods in Anaerobic Bacteriology.  CDC Laboratory Manual.  USDHEW C. D. C. Atlanta, GA 30333.

 

2.        Engelkirk, P. G., Duben-Engelkirk, J., Dowell, V. R.  1992Principles of Practices of Clinical Anaerobic BacteriologyStar Publishing Co., Belmont, CA 94002.

 

3.        Holdeman, L. V., F. P. Cato and W. E. C. Moore.  1977Anaerobe Laboratory ManualVirginia Polytechnic Institute and State University.  Blacksburg, VA 24061.

 

4.        Slots, J.  1982.  Selective Medium for Isolation of Actinobacillus actinomycetemcomitansJournal of Clinical MicrobiologyApril 1982, p. 606 – 609.

 

5.        Somer-Jousimies, H. R., Summanen, P., Citron, D. M., Baron, E. J., Wexler, H. M. and S. M. Finegold.  2002.  Wadsworth – KTL Anaerobic Bacteriology ManualStar Publishing Co., Belmont, CA 94002.

 

6.        NCCLSQuality Control for Commercially Prepared Microbiological Culture Media; Approved Standard- Third Edition(2004).  NCCLS document M22-A3.  NCCLS, 940 West Valley Road, Suite 1400, Wayne, PA 19087-1898.

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