We constructed a cDNA expression construct of the mouse ABO gene and determined specificity. We also constructed the Human-mouse chimeric construct by replacing the last coding exon of the human construct with the mouse counterpart. Four constructs were used as controls: no DNA, pA(arg), pAAAA, and pBBBB. The pA(arg) construct encodes A transferase, except that codon 268 was replaced from glycine to arginine. Due to the amino acid substitution, this construct cannot exhibit A transferase activity, and therefore was used as a negative control. pAAAA and pBBBB constructs encode functional A and B transferases, respectively. Therefore, they were used for positive controls. These constructs were transfected to the HeLa cell, the human uterine cancer cells, and the appearance of A and B antigens were monitored by the immunological method using anti-A and anti-B antibodies. Both the pHuman-mouse and pMouse constructs induced the expression of A and B antigens, suggesting that the mouse gene is a cis-AB gene and encodes an enzyme with both A and B transferase activity.
Histo-blood group ABO system, blood group ABO system, ABO system, AB0 system, ABO blood groups, AB0 blood groups, ABO blood types, AB0 blood types, ABO genetic locus, ABO genes, ABO, AB0, A glycosyltransferases, B glycosyltransferases, glycosyltransferases, A transferase, B transferase, cell surface antigens, carbohydrate antigens, oligosaccharide antigens, oligosaccharides, complex carbohydrate antigens, complex carbohydrates, A antigen, B antigen, H antigen, red blood cell antigens, A/B antigens, ABH antigens, glycolipid, glycosphingolipids, glycoproteins, oligo sugars, red blood cells, RBC, blood transfusion, transfusion medicine, cell/tissue/organ transplantation, transplantation medicine, immunohematology, immunohaematology, immuno-hematology, immunology, ABO genotyping, forensic sciences, legal medicine, human genetics, population genetics, evolution, enzymology, glycobiology, glycosciences, human genes, primate genes, mouse gene, pig genes, alpha 1,3-Gal(NAc) transferases, a1,3-galactosyl transferase, a1,3-GalNAc transferase, structural basis, molecular genetic basis of ABO, ABO polymorphism, single nucleotide polymorphism, SNP, A, B, AB, O, A2, A3, Ax, B3, alleles, weak subgroups, homo sapiens, pig AO genes, cis-AB, B(A), mouse cis-AB gene, ABO genotype, ABO phenotype, DNA methylation, transcription, alternative splicing, Golgi apparatus, transferase chimeras, GBGT1, GGTA1, A3GALT2, monoclonal antibody, sera, plant lectins, Fumi-ichiro Yamamoto, Fumiichiro Yamamoto, F. Yamamoto, Landsteiner, enzyme, kinetics, sugar specificity, acceptor substrate specificity, acceptors, donors, sugars, nucleotide-sugars, genetic engineering, differential susceptibility to infectious diseases, differential cancer susceptibility, alterations in glycosylation in cancer, pancreatic cancer, diets, Peter D'Adamo, Blood type diets, neurobiology, Masahiko Nomi, personality, Burnham Institute, Burnham Institute for Medical Research, Biomembrane Institute, IMPPC, IMPPC Institute of Predictive and Personalized Medicine of Cancer, Institut de Medicina Predictiva i Personalitzada del Càncer, AABB, ISBT, dbRBC - Blood Group Antigen Gene Mutation Database