Finally, when the last 2 amino acids were M of B transferase and G of A transferase, the constructs strongly expressed both A and B antigens. This became the first demonstration where we successfully modified the specificities of glycosyltransferases by genetic engineering and created novel enzymes with both strong A and B transferase activities.
F., and Hakomori, S. (1990). Sugar-nucleotide donor specificity of
histo-blood group A and B transferases is based on amino acid
substitutions. J Biol Chem 265, 19257-19262.
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