An expedition to international waters off the coast of Brazil used state-of-the-art imaging systems to confirm new midwater species and observed the living 3D cellular structure of a microbe — a first for seagoing research.
Fortaleza, Brazil— An international team of midwater experts on board Schmidt Ocean Institute’s research vessel Falkor (too) discovered over two dozen new marine species on a recent expedition off the coast of Brazil in the tropical South Atlantic Ocean. The scientists used advanced technologies to explore the Ocean’s midwater — the water between the sunlit layer and the seafloor — which is Earth’s largest and least explored habitable ecosystem. It can take scientists decades to identify and describe new species, but the combination of technology and expertise enabled the team to confirm these species as new within a matter of days.
This is a new species from the genus Tomopteris, commonly known as gossamer worms. The expedition science team tested new technology that provides scientists with new, non-invasive ways to study these remarkable animals. Credit: ROV SuBastian / Schmidt Ocean Institute
The list consists of an amphipod, a type of crustacean related to crabs and lobsters; a gossamer worm that moves faster than scientists expect it to based on its body shape; nine jellyfish; seven siphonophores, colonial organisms related to jellyfish and corals; seven comb jellies or ctenophores, famous for the glittering cilia they use to swim; four larvaceans, tadpole-like creatures that live in mucus houses and are more closely related to humans than invertebrates; and two giant rhizarians, single-celled organisms visible to the naked eye.
“The largest habitat on Earth, the midwater, is filled with incredible animals we are only just starting to understand,” said the expedition’s chief scientist, Dr. Karen Osborn of the Smithsonian National Museum of Natural History. “I continue to be fascinated by the fantastic variety of solutions they have evolved to survive in this formidable environment, and that drives me to keep asking questions about our ocean.”
Expedition Chief Scientist Dr. Karen Osborn (Smithsonian National Museum of Natural History) works with Dr. Heather Judkins (University of South Florida, St. Petersburg) and Dr. Silvina Botta (Universidade Federal do Rio Grande) in the wet lab of R/V Falkor (too), gathering tiny translucent animals from a large container for further study. Credit: Alex Ingle / Schmidt Ocean Institute
The team witnessed far more diversity and abundance of midwater organisms than they expected, said Osborn, including glass squid and a pelagic octopus feeding on a bright red jellyfish.
A female octopus (Haliphron atlanticus) consumes a jellyfish at 800 meters depth. Observing feeding interactions helps scientists understand how midwater communities function and offers insights into processes we all depend on, such as carbon cycling through the largest habitat on earth. Credit: ROV SuBastian / Schmidt Ocean Institute
The Ocean’s midwater is one of the most challenging areas on Earth to explore because of its inaccessibility and immense volume. The Sasakawa Peace Foundation’s Ocean Shot Research Grant Program funded two midwater programs that made this work possible, one based at the University of Western Australia and the other at Bigelow Laboratory for Ocean Sciences, USA.
Principal Investigator Dr. John Burns (Bigelow Laboratory for Ocean Sciences) examines a sample, which will be used for his genetic sequencing work in the main lab of R/V Falkor (too). In tandem with high-resolution imagery gathered at depth, the team sequenced genomes from collected specimens onboard the vessel, enabling them to rapidly identify new species. Credit: Alex Ingle / Schmidt Ocean Institute
The technologies used to identify new species were a combination of imaging systems and genetic analyses.
The imaging systems included the DeepPIV (particle image velocimetry) and EyeRIS (remote imaging system) instruments, developed by the Bioinspiration Lab at MBARI (Monterey Bay Aquarium Research Institute), which were attached to Schmidt Ocean Institute’s remotely operated vehicle (ROV) SuBastian. DeepPIV and EyeRIS are sophisticated, non-invasive tools for scanning marine animals; they use lasers to scan organisms and create 3D images of them. In addition, the team attached a shadowgraph camera from the Japan Agency for Marine-Earth Science and Technology (JAMSTEC) to the ROV, which can image the finer details of animals not visible in the 3D scans. The images help scientists describe the shape and internal structures of animals without having to collect them.
This juvenile glass squid, collected by ROV SuBastian at 779 meters depth in the South Atlantic, was photographed on R/V Falkor (too) using a prototype multiview macro camera system
Credit: Emily Clark / MBARI
This image captured by a shadowgraph camera shows the front, side and top views of protective shield tissues from a siphonophore.
Credit: Dhugal Lindsay / JAMSTEC
A microscopic view shows the complex, honeycomb-like structure of a hyperiid’s eye, a tiny shrimp-like crustacean that lives in the deep ocean. Researchers used advanced 3D confocal live-imaging technology to capture individual cone cells that form the creature's compound eyes. Credit: Manu Prakash / Stanford University
“It’s an incredible honor to not only view and experience this rare and inspiring midwater life, but also to be able to work towards describing and sharing that life broadly through the use of novel, non-invasive technologies,” said Dr. Kakani Katija, principal engineer of the Bioinspiration Lab at MBARI.
A siphonophore — a colonial marine invertebrate related to the venomous stinging Portuguese Man-o-war (Physalia physalis) — is scanned using the Deep Particle Image Velocimetry (DeepPIV) at a depth of 350 meters. The imaging system was developed by the Bioinspiration Lab at MBARI (Monterey Bay Aquarium Research Institute) to create 3D models of gelatinous animals. This species was undescribed prior to this encounter and is likely new to science. Credit: ROV SuBastian / Schmidt Ocean Institute
Many midwater animals are gelatinous, with soft, delicate bodies that are often damaged by traditional sampling methods. To address this challenge, the expedition used additional technologies that allowed scientists to observe animals in a controlled environment that mimics their natural habitat. These included a virtual reality chamber developed at the University of Western Australia and a “gravity machine” developed at Stanford University — a specialized microscope that functions as a hydrodynamic treadmill for studying microbes.
(L-R) Manu Prakesh (Stanford University) and Lakshmi Swaminathan (University of Western Australia) transfer animals to experimental imaging systems in a laboratory on research vessel Falkor (too).
Credit: Alex Ingle / Schmidt Ocean Institute
This is a phase-contrast image of a live phyllosoma, a larval stage of a spiny or slipper lobster (magnified 10x). The microscopic details of the animal’s completely transparent digestive and nervous systems — tightly sandwiched between its ultra-thin, transparent exoskeleton — are visible in this image. Credit: Manu Prakash / Stanford University
This jelly was collected at 1157 meters depth by ROV SuBastian and photographed on board Falkor (too) using a prototype Multiview Macro Camera system.
Credit: Emily Clark / MBARI
The team used another microscope developed at Stanford University to gain critical new insights into the physiology of midwater animals. The microscope, known as Squid, is an open-source, confocal microscope. Using Squid, the team achieved a first for research at sea and imaged living internal cellular structures in 3D. One of the organisms imaged was a large single-celled microbe called a protist. The microscope enabled the scientists to observe how the protist’s cellular structure interacted with its glass skeleton.
Dr. Silvina Botta (Universidade Federal do Rio Grande in Brazil) retrieves water samples from ROV SuBastian for stable isotope analysis — a chemical way to study how carbon moves through the ocean. Credit: Alex Ingle / Schmidt Ocean Institute
“This opens a new door for researching deep-sea physiology, linking cellular architectures to organism function. We can now witness live internal processes within these extreme organisms adapted to withstand immense pressure and darkness,” said Dr. Manu Prakash of Stanford University.
Dr. Manu Prakash (Stanford University) at work in the Main Lab on R/V Falkor (too). Prakash’s lab developed a confocal microscope, used extensively during the expedition, that provides image data to map out the complex, three-dimensional internal structures of tiny sea creatures. Credit: Alex Ingle / Schmidt Ocean Institute
In tandem with the high-resolution imagery, the team sequenced genomes from collected specimens onboard the vessel, enabling them to rapidly identify new species under the leadership of Dr. Cheryl Ames of Tohoku University and Dr. John Burns of Bigelow Laboratory.
Remotely Operated Vehicle (ROV) SuBastian is deployed from the research vessel Falkor (too) for a research dive in the South Atlantic during the expedition. Credit: Alex Ingle / Schmidt Ocean Institute
“The novel suite of technologies on this cruise is a glimpse into the future of marine biological science,” said Schmidt Ocean Institute’s Executive Director, Dr. Jyotika Virmani. “Schmidt Ocean Institute’s mission is to push technological advancement and this was our third cruise in collaboration with this team of scientists and engineers to test and further develop this innovative midwater equipment. We look forward to a future in which scientists study marine life as elegantly as this team did - and in virtual reality.”
Learn more about the previous two Designing the Future expeditions:
https://schmidtocean.org/cruise/designing-the-future/
https://schmidtocean.org/cruise/designing-the-future-2/
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About the Organizations
Schmidt Ocean Institute was established in 2009 by Eric and Wendy Schmidt to catalyze the discoveries needed to understand our ocean, sustain life, and ensure the health of our planet through the pursuit of impactful scientific research and intelligent observation, technological advancement, open sharing of information, and public engagement, all at the highest levels of international excellence. For more information, visit www.schmidtocean.org.
The National Museum of Natural History is connecting people everywhere with Earth’s unfolding story. It is one of the most visited natural history museums in the world. Opened in 1910, the museum is dedicated to maintaining, preserving, and making accessible the world’s most extensive collection of natural history specimens and human artifacts. For more information, visit the museum on its website, blog, Facebook, LinkedIn and Instagram.
Bigelow Laboratory for Ocean Sciences is an independent, nonprofit research institute located in East Boothbay, Maine. From the Arctic to the Antarctic, Bigelow Laboratory scientists use innovative approaches to study the foundation of global ocean health and unlock its potential to improve the future for all life on our planet. Learn more at https://www.bigelow.org/
MBARI (Monterey Bay Aquarium Research Institute) is a non-profit oceanographic research center founded in 1987 by the late Silicon Valley innovator and philanthropist David Packard. Our mission is to advance marine science and engineering to understand our changing ocean. Learn more at mbari.org.
For over 110 years, The University of Western Australia has been a place where change is seen as an opportunity. As Western Australia’s first university and a member of the prestigious Group of Eight, we don’t just honour our history, we use it as a launchpad for the future, empowering our community to turn ambition into real-world impact. Learn more at https://www.uwa.edu.au/home