B4.3.2.1

Producing monoclonal antibodies

Content

    • Students should be able to describe how monoclonal antibodies are produced.

Monoclonal antibodies are produced from a single clone of cells. The antibodies are specific to one binding site on one protein antigen and so are able to target a specific chemical or specific cells in the body.

They are produced by stimulating mouse lymphocytes to make a particular antibody. The lymphocytes are combined with a particular kind of tumour cell to make a cell called a hybridoma cell. The hybridoma

cell can both divide and make the antibody. Single hybridoma cells

are cloned to produce many identical cells that all produce the same antibody. A large amount of the antibody can be collected and purified.